CAJ | 학술논문

采用高效液相色谱-三重四极杆质谱（ HPLC-TQMS），建立了同时分析羊栖菜主分枝、侧分枝、初生叶、次生叶、茎等5个部位中吲哚乙酸、异戊烯腺苷、异戊烯腺嘌呤、反式玉米素核苷、玉米素、独角金内酯、脱落酸、水杨酸、赤霉素、茉莉酸等10种植物激素含量的方法。羊栖菜样品经冷冻干燥后，用甲醇/水/甲酸（15：4：1，v / v / v）（含0.5％ BHT）超声提取植物激素，在 Hypersil Gold C18色谱柱上，以甲醇和水为流动相进行梯度洗脱，采用电喷雾电离，正/负离子扫描选择反应监测模式进行定量分析。结果表明，10种植物激素在线性范围内的相关系数（ r）为0.9989~1.0000；检出限为0.0012~4.6512μg / L；加标回收率在72.24％~91.31％之间，相对标准偏差均不高于6.59％；在羊栖菜5个部位鲜样中10种植物激素的含量范围为未检出至4041.431 ng / g。本方法具有良好的灵敏度、精密度和回收率，可以用于同时分析多种植物激素。
채용고효액상색보-삼중사겁간질보（ HPLC-TQMS），건립료동시분석양서채주분지、측분지、초생협、차생협、경등5개부위중신타을산、이무희선감、이무희선표령、반식옥미소핵감、옥미소、독각금내지、탈락산、수양산、적매소、말리산등10충식물격소함량적방법。양서채양품경냉동간조후，용갑순/수/갑산（15：4：1，v / v / v）（함0.5％ BHT）초성제취식물격소，재 Hypersil Gold C18색보주상，이갑순화수위류동상진행제도세탈，채용전분무전리，정/부리자소묘선택반응감측모식진행정량분석。결과표명，10충식물격소재선성범위내적상관계수（ r）위0.9989~1.0000；검출한위0.0012~4.6512μg / L；가표회수솔재72.24％~91.31％지간，상대표준편차균불고우6.59％；재양서채5개부위선양중10충식물격소적함량범위위미검출지4041.431 ng / g。본방법구유량호적령민도、정밀도화회수솔，가이용우동시분석다충식물격소。
A method for the simultaneous determination of indole-3-acetic acid,N 6-(2-isopen-tenyl) adenosine,N 6-( 2-isopentenyl) adenine,trans-zeatin riboside,zeatin,strigolactone, abscisic acid,salicylic acid,gibberellin A3 and jasmonic acid in five different parts of main branch,lateral branch,primary leaf,secondary leaf and stem of Sargasum fusiforme(Hary.) Seichell was established by high performance liquid chromatography-triple quadrupole mass spectrometry( HPLC-TQMS). The samples were extracted with methanol / water / formic acid (15 : 4 : 1,v / v / v )( containing 0. 5% 2,6-di-tert-butyl-4-methylphenol,BHT ) after vacuum freeze-drying. The separation was performed on a Hypersil Gold C18 column by using methanol and water as mobile phases with gradient elution. The analytes were detected by tandem mass spectrometry in selected reaction monitoring( SRM)mode. The electrospray ionization( ESI) source was used for the quantitative analysis in the positive mode or negative mode. Under the optimized conditions,the correlation coefficients( r) of the ten phytohormones were from 0. 998 9 to 1. 000 0 in the linear ranges. The detection limits of the ten phytohormones were 0. 001 2-4. 651 2 μg / L. The average recoveries were 72. 24% -91. 31% with the relative standard deviations not more than 6. 59% . In the five parts of fresh Sargasum fusiforme(Hary.)Seichell samples,the contents of the ten phytohormones were from not detected to 4 041. 431 ng / g. This method has good sensitivity,precision,recovery,and can be used to simultaneously determine the phytohormones.