CAJ | 학술논문

目的：大豆黄酮( Daidzein)玻璃体腔内注射是否对视神经急性损伤有保护和修复作用。 　　方法：SD雄性大鼠72只,建立大鼠急性视神经钳夹损伤模型,随机分为正常组、PBS阴性对照组、大豆黄酮治疗组(10μmol/L,100μmol/L,1000μmol/L)、鼠神经生长因子(mNGF,100ng/mL)阳性对照组；玻璃体内注射3d后处死各组大鼠,用组织病理( HE )、免疫组化检测以及Western Blot分析来观察和评价视网膜形态变化及进行各组间视网膜 Caspase-3蛋白和 GAP-43蛋白表达的比较。 　　结果：与正常组和阴性对照组相比,不同浓度的大豆黄酮各治疗组和阳性对照组的视网膜形态结构较完整, Caspase-3蛋白的表达相对降低,GAP-43蛋白的表达相对较高,其差异有统计学意义(P<0.05)。 　　结论：大豆黄酮玻璃体腔注射对大鼠急性视神经损伤具有修复和保护作用。
목적：대두황동( Daidzein)파리체강내주사시부대시신경급성손상유보호화수복작용。 　　방법：SD웅성대서72지,건립대서급성시신경겸협손상모형,수궤분위정상조、PBS음성대조조、대두황동치료조(10μmol/L,100μmol/L,1000μmol/L)、서신경생장인자(mNGF,100ng/mL)양성대조조；파리체내주사3d후처사각조대서,용조직병리( HE )、면역조화검측이급Western Blot분석래관찰화평개시망막형태변화급진행각조간시망막 Caspase-3단백화 GAP-43단백표체적비교。 　　결과：여정상조화음성대조조상비,불동농도적대두황동각치료조화양성대조조적시망막형태결구교완정, Caspase-3단백적표체상대강저,GAP-43단백적표체상대교고,기차이유통계학의의(P<0.05)。 　　결론：대두황동파리체강주사대대서급성시신경손상구유수복화보호작용。
AIM:To discuss Daidzein intravitreal injection whether has protective and recovery effects on acute nerve damages. METHODS:After the crush models of acute optic nerve were set up, 72 males SD rats were divided into 4 groups randomly as common group without surgery, FBS negative control group, Daidzein treatment group ( 10μmol/L, 100μmol/L, 1000μmol/L ) and positive control group using rats nerve growth factor ( mNGF, 100ng/mL ). Three days after interference, all experimental animals were executed. HE staining was used to evaluate morphologic change of the retina, immunohisochemical staining and western-blot tests for identifying and quantifying the distinct expression of Caspase-3 and GAP-43 among the groups. RESULTS: Compared with the normal group and negative control group, retinal morphology of different concentrations of each Daidzein treatment group and positive control group was more complete, the expression of Caspase-3 protein was relatively lower, the expression of GAP-43 protein was relatively higher, the differences have statistically significance (P<0. 05).CONCLUSION: Daizein injection in the vitreous cavity has the capacity of protection and restoration in rat's acute nerve damages.