临床肝胆病杂志
臨床肝膽病雜誌
림상간담병잡지
CHINESE JOURNAL OF CLINICAL HEPATOLOGY
2014年
8期
752-756
,共5页
席鹏程%时开网%朱响%杨坤兴%刘子君%卞建民%赵有财
席鵬程%時開網%硃響%楊坤興%劉子君%卞建民%趙有財
석붕정%시개망%주향%양곤흥%류자군%변건민%조유재
胰腺肿瘤%血管内皮生长因子A%尿纤溶酶原激活物%基因表达
胰腺腫瘤%血管內皮生長因子A%尿纖溶酶原激活物%基因錶達
이선종류%혈관내피생장인자A%뇨섬용매원격활물%기인표체
pancreatic neoplasms%vascular endothelial growth factor A%urinary plasminogen activator%gene expression
目的：探讨胰腺癌组织中血管内皮生长因子（VEGF）mRNA、尿激酶型纤溶酶原激活物（uPA）mRNA定量表达的临床意义。方法自2008年1月至2011年12月于本科行胰头癌根治术的患者中筛选出经病理证实为导管腺癌的30例患者的完整资料，采用荧光定量PCR（qPCR）检测其胰腺癌组织及6例正常胰腺组织中VEGF mRNA、uPA mRNA定量表达，分析其与临床病理因素之间的关系。结果 VEGF mRNA、uPA mRNA的表达与胰腺癌的组织分化程度、神经侵犯有关。VEGF mRNA在淋巴结转移阳性组中的定量表达高于淋巴结转移阴性组，两组比较差异有统计学意义（t＝20．007，P＝0．000）；uPA mRNA在直径≤2 cm的肿瘤组织中定量水平小于直径＞2 cm的肿瘤组织，两组比较差异有统计学意义（t＝7．539，P＝0．000）。uPA mRNA在伴有十二指肠侵犯组中的定量表达高于无十二指肠侵犯组，两组比较差异有统计学意义（t＝-2．089，P＝0．037）。uPA mRNA在Ⅲ期肿瘤组织中的定量表达高于Ⅰ、Ⅱ期肿瘤组织中的定量表达，两组比较差异有统计学意义（t＝-9．450，P＝0．000）。VEGF mRNA与uPA mR-NA的表达存在正相关（r＝0．334，P＝0.000）。结论 VEGF mRNA、uPA mRNA在胰腺癌组织中过度表达可为肿瘤细胞的侵润创造条件。
目的：探討胰腺癌組織中血管內皮生長因子（VEGF）mRNA、尿激酶型纖溶酶原激活物（uPA）mRNA定量錶達的臨床意義。方法自2008年1月至2011年12月于本科行胰頭癌根治術的患者中篩選齣經病理證實為導管腺癌的30例患者的完整資料，採用熒光定量PCR（qPCR）檢測其胰腺癌組織及6例正常胰腺組織中VEGF mRNA、uPA mRNA定量錶達，分析其與臨床病理因素之間的關繫。結果 VEGF mRNA、uPA mRNA的錶達與胰腺癌的組織分化程度、神經侵犯有關。VEGF mRNA在淋巴結轉移暘性組中的定量錶達高于淋巴結轉移陰性組，兩組比較差異有統計學意義（t＝20．007，P＝0．000）；uPA mRNA在直徑≤2 cm的腫瘤組織中定量水平小于直徑＞2 cm的腫瘤組織，兩組比較差異有統計學意義（t＝7．539，P＝0．000）。uPA mRNA在伴有十二指腸侵犯組中的定量錶達高于無十二指腸侵犯組，兩組比較差異有統計學意義（t＝-2．089，P＝0．037）。uPA mRNA在Ⅲ期腫瘤組織中的定量錶達高于Ⅰ、Ⅱ期腫瘤組織中的定量錶達，兩組比較差異有統計學意義（t＝-9．450，P＝0．000）。VEGF mRNA與uPA mR-NA的錶達存在正相關（r＝0．334，P＝0.000）。結論 VEGF mRNA、uPA mRNA在胰腺癌組織中過度錶達可為腫瘤細胞的侵潤創造條件。
목적：탐토이선암조직중혈관내피생장인자（VEGF）mRNA、뇨격매형섬용매원격활물（uPA）mRNA정량표체적림상의의。방법자2008년1월지2011년12월우본과행이두암근치술적환자중사선출경병리증실위도관선암적30례환자적완정자료，채용형광정량PCR（qPCR）검측기이선암조직급6례정상이선조직중VEGF mRNA、uPA mRNA정량표체，분석기여림상병리인소지간적관계。결과 VEGF mRNA、uPA mRNA적표체여이선암적조직분화정도、신경침범유관。VEGF mRNA재림파결전이양성조중적정량표체고우림파결전이음성조，량조비교차이유통계학의의（t＝20．007，P＝0．000）；uPA mRNA재직경≤2 cm적종류조직중정량수평소우직경＞2 cm적종류조직，량조비교차이유통계학의의（t＝7．539，P＝0．000）。uPA mRNA재반유십이지장침범조중적정량표체고우무십이지장침범조，량조비교차이유통계학의의（t＝-2．089，P＝0．037）。uPA mRNA재Ⅲ기종류조직중적정량표체고우Ⅰ、Ⅱ기종류조직중적정량표체，량조비교차이유통계학의의（t＝-9．450，P＝0．000）。VEGF mRNA여uPA mR-NA적표체존재정상관（r＝0．334，P＝0.000）。결론 VEGF mRNA、uPA mRNA재이선암조직중과도표체가위종류세포적침윤창조조건。
Objective To investigate the clinical significance of quantitative expression of vascular endothelial growth factor (VEGF)mR-NA and urokinase-type plasminogen activator (uPA)mRNA in pancreatic cancer tissue.Methods A retrospective study was conducted on the complete data of 30 patients with a pathological diagnosis of duct adenocarcinoma who were selected from those treated by radical re-section of pancreatic head carcinoma from January 2008 to December 2011.Real-time quantitative PCR was used to measure the quantita-tive expression of VEGF mRNA and uPA mRNA in the pancreatic cancer tissues of the 30 cases and the normal pancreatic tissues of 6 con-trols,and its relationship with clinicopathological factors was analyzed.Results The quantitative expression of VEGF mRNA and uPA mR-NA was correlated with the histological differentiation and perineural invasion of pancreatic cancer.The quantitative expression of VEGF mR-NA was significantly higher in patients with lymphatic metastasis than in those without lymphatic metastasis group (t=20.007,P=0.000). The quantitative expression of uPA mRNA was significantly lower in the tumors with a diameter of≤2 cm than in the tumors with a diameter of >2 cm (t=7.539,P=0.000).Patients with duodenal invasion had a significantly higher quantitative expression of uPA mRNA than those without duodenal invasion (t=-2.089,P=0.037).The quantitative expression of uPA mRNA in stage III tumor tissues was signifi-cantly higher than that of uPA mRNA in stage I and II tumor tissues (t=-9.450,P=0.000).There was a positive correlation between VEGF mRNA expression and uPA mRNA expression (r=0.334,P=0.000).Conclusion The overexpression of VEGF mRNA and uPA mRNA in pancreatic cancer tissue may create an environment that enables the invasion by pancreatic cancer cells.
