CAJ | 학술논문

采用乙醇/(NH4)2SO4两相盐析体系，从酿酒副产物葡萄籽中提取多酚、类黄酮和原花青素。实验结果表明，在室温下用组成（质量分数）为30%乙醇/18%(NH4)2SO4的体系提取葡萄籽中多酚、类黄酮以及原花青素，得率分别为64.54、42.45、23.16 mg·g-1；它们富集于上相乙醇相，萃取回收率分别为97.31%、98.19%、97.92%。室温条件下用2,2-二苯基-1-苦基肼（DPPH）法与2,2′-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)（ABTS）法测定两相盐析萃取物抗氧化、清除自由基能力，IC50分别为61.85、71.76 mg·L-1，比传统的热回流提取物具有更强的抗氧化活性。
채용을순/(NH4)2SO4량상염석체계，종양주부산물포도자중제취다분、류황동화원화청소。실험결과표명，재실온하용조성（질량분수）위30%을순/18%(NH4)2SO4적체계제취포도자중다분、류황동이급원화청소，득솔분별위64.54、42.45、23.16 mg·g-1；타문부집우상상을순상，췌취회수솔분별위97.31%、98.19%、97.92%。실온조건하용2,2-이분기-1-고기정（DPPH）법여2,2′-련담기-쌍-(3-을기분병이경새서람-6-광산)（ABTS）법측정량상염석췌취물항양화、청제자유기능력，IC50분별위61.85、71.76 mg·L-1，비전통적열회류제취물구유경강적항양화활성。
Salting-out extraction system composed of ethanol and ammonium sulfate was used to separate total phenolics, flavonoid, and proanthocyanidin from wine grape seed. The experimental results showed that components were extracted into the top ethanol-rich phase (the system consisted of 30% (mass) ethanol/18%(mass) ammonium sulfate) to give the yield of total phenolics, flavonoid, and proanthocyanidin in 64.54, 42.45, and 23.16 mg·g-1, and corresponding recovery rates in 97.31%, 98.19%, and 97.92%, respectively. The antioxidant activity of seed extracts were assayed with radical-scavenging ability (IC50) of 2,2-diphenyl-1-picrylhydrazyl and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) at room temperature. The seed extracts by salting-out extraction showed lower IC50 (61.85 and 71.76 mg·L-1) than that by heat reflux extraction (78.73 and 90.34 mg·L-1).