CAJ | 학술논문

目的：探讨单采血小板与混合血小板在血液采集制备过程中细菌污染的危险程度，为血小板的采集制备过程中改良工艺，提高输血安全性提供可靠数据。方法采用生物梅里埃公司生产的BaeT/Alert 3D 全自动细菌培养仪及应用需氧瓶（BPA Culture Bottle）、厌氧瓶（BPN Culture Bottle）对5210例单采血小板，1653例混合血小板进行了细菌培养，细菌培养阳性者转种，转种阳性者进行细菌鉴定。结果在5210例单采血小板细菌培养经确定阳性6例，阳性率为1.15‰；1653例混合血小板中细菌培养经确定阳性10例，阳性率6.05‰。结论混合血小板细菌培养阳性率（6.05‰）高于单采血小板（1.15‰），两者比较差异有统计学意义（P＜0.01）；血小板细菌污染情况仍然严峻，应引起注意。
목적：탐토단채혈소판여혼합혈소판재혈액채집제비과정중세균오염적위험정도，위혈소판적채집제비과정중개량공예，제고수혈안전성제공가고수거。방법채용생물매리애공사생산적BaeT/Alert 3D 전자동세균배양의급응용수양병（BPA Culture Bottle）、염양병（BPN Culture Bottle）대5210례단채혈소판，1653례혼합혈소판진행료세균배양，세균배양양성자전충，전충양성자진행세균감정。결과재5210례단채혈소판세균배양경학정양성6례，양성솔위1.15‰；1653례혼합혈소판중세균배양경학정양성10례，양성솔6.05‰。결론혼합혈소판세균배양양성솔（6.05‰）고우단채혈소판（1.15‰），량자비교차이유통계학의의（P＜0.01）；혈소판세균오염정황잉연엄준，응인기주의。
Objective To investigate the criticality of bacterial pollution in the blood collection and preparation process of single donor platelets and mixed platelets in order to provide reliable data for improving the technology in the collection and preparation process of platelets and improving the blood transfusion safety. Methods The BaeT/Alert 3D Full-Automatic Bacterial Culture System produced by BioMérieux and the BPA Culture Bottle and BPN Culture Bottle were used for the bacterial culture of 5210 cases of single donor platelets and 1653 cases of mixed platelets.The samples showing g positive bacterial culture results received subcultivation and the subcultivated sample showing positive results received bacterial identification. Results Of the 5210 cases of single donor platelets,bacterial culture confirmed 6 positive cases,with the positive rate of 1.15‰.Of the 1653 cases of mixed platelets,bacterial culture confirmed 10 positive cases,with the positive rate of 6.05‰. Conclusion The positive bacterial culture rate of mixed platelets (6.05‰)is higher than that of the single donor platelets (1.15‰), with statistically significant difference (P<0.01).The bacterial pollution situation of platelets is still severe and needs attention.