CAJ | 학술논문

目的：探讨丁酸钠对乳腺癌细胞MCF-7生长的抑制作用。方法将乳腺癌细胞MCF-7常规培养至对数生长期，采用浓度为0、2.5、5.0、10.0mmol/L的丁酸钠处理后，观察对MCF生长的抑制作用。结果浓度为2.5、5.0、10.0mmol/L的丁酸钠处理的MCF-7细胞生长速度明显慢于亲本细胞，随着浓度的增加呈减慢趋势，差异有统计学意义（P＜0.05）。浓度为5mmol/L的丁酸钠处理后的MCF-7细胞克隆形成率明显低于MCF亲本细胞，差异有统计学意义（P＜0.05）。丁酸钠处理的细胞G1明显高于亲本细胞，S期及G2/M期明显低于亲本细胞，凋亡细胞百分比明显高于亲本细胞，差异有统计学意义（P＜0.05）。结论丁酸钠可能是通过诱导MCF-7细胞凋亡而起到逆转其恶性生物学行为的作用。
목적：탐토정산납대유선암세포MCF-7생장적억제작용。방법장유선암세포MCF-7상규배양지대수생장기，채용농도위0、2.5、5.0、10.0mmol/L적정산납처리후，관찰대MCF생장적억제작용。결과농도위2.5、5.0、10.0mmol/L적정산납처리적MCF-7세포생장속도명현만우친본세포，수착농도적증가정감만추세，차이유통계학의의（P＜0.05）。농도위5mmol/L적정산납처리후적MCF-7세포극륭형성솔명현저우MCF친본세포，차이유통계학의의（P＜0.05）。정산납처리적세포G1명현고우친본세포，S기급G2/M기명현저우친본세포，조망세포백분비명현고우친본세포，차이유통계학의의（P＜0.05）。결론정산납가능시통과유도MCF-7세포조망이기도역전기악성생물학행위적작용。
Objective To investigate the inhibitory effect of sodium butyrate on the growth of breast cancer cells MCF-7. Methods Breast cancer MCF-7 cells were cultured to logarithmic growth phase, they were processed with sodium butyrate, 0, 2.5, 5.0, 10.0mmol/L were the concentration, the growth inhibition of MCF was observed. Results The growth rate of MCF-7 cell processed with the sodium butyrate with the Concentration 2.5, 5.0, 10.0mmol/L was significantly slower than that of the parent cell, the growth rate with increasing concentration was slower, there was the significant difference(P < 0.05). The colony efficiency of MCF-7 cells processed with the sodium butyrate with the concentration 5mmol/L was significantly lower than that of the parental cells, there was the significant difference (P < 0.05). After processed with sodium butyrate, Cell G1 was significantly higher than the parental cells, S phase and G2 / M phase were significantly lower than the parental cells, the percentage of apoptotic cells was significantly higher than the parental cells, there was the significant difference(P<0.05). Conclusion Sodium butyrate may be by inducing apoptosis in MCF-7 cells and play a reversal of its role in the malignant behavior.