中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
11期
30-32
,共3页
小鼠%无精子症%动物模型%效果
小鼠%無精子癥%動物模型%效果
소서%무정자증%동물모형%효과
Mouse%Azoospermatism%Animal model%Effect
目的：分析稳定的小鼠无精子症动物模型构建的方法。方法选取60只清洁级C5BL/6小鼠，将小鼠按照编号，随机分为两组，化疗组和激素组各30只。分别观察药物注射后，小鼠睾丸生精小管内精子和精子细胞消失情况。结果化疗组小鼠在接受药物注射后第4周，睾丸生精小管内精子及精子细胞消失，化疗后20周仍未产生精子，激素组在经过苯甲酸雌二醇连续注射后，于第4周睾丸生精小管内精子以及精子细胞完全消失，连续注射第20周时，部分小鼠睾丸生精小管内发现了少量的精子细胞与精子存在。两组小鼠睾丸重量存在显著差异性，具备统计学意义（P＜0.05）。结论激素组采用苯甲酸雌二醇注射构建的小鼠无精子症动物模型缺乏稳定性，化疗组经过白消安、环磷酰胺注射后构建的小鼠无精子症模型较为稳定，是构建小鼠无精子症动物模型的首选方法，值得进行推广应用。
目的：分析穩定的小鼠無精子癥動物模型構建的方法。方法選取60隻清潔級C5BL/6小鼠，將小鼠按照編號，隨機分為兩組，化療組和激素組各30隻。分彆觀察藥物註射後，小鼠睪汍生精小管內精子和精子細胞消失情況。結果化療組小鼠在接受藥物註射後第4週，睪汍生精小管內精子及精子細胞消失，化療後20週仍未產生精子，激素組在經過苯甲痠雌二醇連續註射後，于第4週睪汍生精小管內精子以及精子細胞完全消失，連續註射第20週時，部分小鼠睪汍生精小管內髮現瞭少量的精子細胞與精子存在。兩組小鼠睪汍重量存在顯著差異性，具備統計學意義（P＜0.05）。結論激素組採用苯甲痠雌二醇註射構建的小鼠無精子癥動物模型缺乏穩定性，化療組經過白消安、環燐酰胺註射後構建的小鼠無精子癥模型較為穩定，是構建小鼠無精子癥動物模型的首選方法，值得進行推廣應用。
목적：분석은정적소서무정자증동물모형구건적방법。방법선취60지청길급C5BL/6소서，장소서안조편호，수궤분위량조，화료조화격소조각30지。분별관찰약물주사후，소서고환생정소관내정자화정자세포소실정황。결과화료조소서재접수약물주사후제4주，고환생정소관내정자급정자세포소실，화료후20주잉미산생정자，격소조재경과분갑산자이순련속주사후，우제4주고환생정소관내정자이급정자세포완전소실，련속주사제20주시，부분소서고환생정소관내발현료소량적정자세포여정자존재。량조소서고환중량존재현저차이성，구비통계학의의（P＜0.05）。결론격소조채용분갑산자이순주사구건적소서무정자증동물모형결핍은정성，화료조경과백소안、배린선알주사후구건적소서무정자증모형교위은정，시구건소서무정자증동물모형적수선방법，치득진행추엄응용。
Objective To analyze the methods of stable mice azoospermatism animal model building. Methods Sixty mice with clean grade C5BL/6 were randomly divided into the chemotherapy group with 30 mice and the hormone group with 30 mice according to the serial number. The sperm and spermoblast disappearance situation in the mice's testicular seminiferous tubules after the drug injection of the two groups was observed. Results In the chemotherapy group, the mice's sperm and spermoblast in the testicular seminiferous tubules disappeared in the 4th week of drug injection,and no sperm was produced in the 20th week of chemotherapy. In the hormone group,after continuous injection of estradiol benzoate,the mice's sperm and spermoblast in the testicular seminiferous tubules disappeared completely in the 4th week,and in the 20th week of continuous injection,little spermoblast and sperm was found in the mice's testicular seminiferous tubules. The two groups of mice had significantly different testical weight,with statistical significance(P < 0.05). Conclusion The mice azoospermatism animal model established by injecting estradiol benzoate in the hormone group lacks stability.The mice azoospermatism animal model established by injecting busulfan and cyclophosphamide in the chemotherapy group is relatively stable,which is the first choice of establishing mice azoospermatism animal model and worthy of promotion and application.
