中国医药科学
中國醫藥科學
중국의약과학
CHINA MEDICINE AND PHARMACY
2014年
12期
11-13
,共3页
侯敬申%侯敬侠%王伟雄%赵莉
侯敬申%侯敬俠%王偉雄%趙莉
후경신%후경협%왕위웅%조리
阿霉素%热化疗%人肝癌细胞%细胞增殖
阿黴素%熱化療%人肝癌細胞%細胞增殖
아매소%열화료%인간암세포%세포증식
Doxorubicin%Heating chemotherapy%Human hepatoma cells%Cell proliferation
目的：探讨阿霉素加热化疗对人肝癌细胞HHCC及HepG2的抑制作用。方法选择体外培养对数生长期HHCC及HepG2细胞为研究对象，采用10%胎牛血清培养液配制成细胞密度5×104/mL的单细胞悬液，接种于50mL的培养瓶中，细胞分为3组，分别进行单纯加热、单纯化疗、加热化疗处理；采用MTT法检测细胞增殖抑制率，采用流式细胞仪检测细胞凋亡率。结果HHCC及HepG2细胞增殖抑制率3组间比较，差异有统计学意义（P＜0.05）；加热化疗组细胞增殖抑制率明显高于单纯加热组及单纯化疗组（P＜0.05）；HHCC及HepG2细胞凋亡率三组间差异有统计学意义（P＜0.05），加热化疗组细胞凋亡率明显高于单纯加热组及单纯化疗组（P＜0.05）。结论加热化疗可明显增强阿霉素对人肝癌细胞HHCC及HepG2的增殖抑制作用，对临床治疗有借鉴意义。
目的：探討阿黴素加熱化療對人肝癌細胞HHCC及HepG2的抑製作用。方法選擇體外培養對數生長期HHCC及HepG2細胞為研究對象，採用10%胎牛血清培養液配製成細胞密度5×104/mL的單細胞懸液，接種于50mL的培養瓶中，細胞分為3組，分彆進行單純加熱、單純化療、加熱化療處理；採用MTT法檢測細胞增殖抑製率，採用流式細胞儀檢測細胞凋亡率。結果HHCC及HepG2細胞增殖抑製率3組間比較，差異有統計學意義（P＜0.05）；加熱化療組細胞增殖抑製率明顯高于單純加熱組及單純化療組（P＜0.05）；HHCC及HepG2細胞凋亡率三組間差異有統計學意義（P＜0.05），加熱化療組細胞凋亡率明顯高于單純加熱組及單純化療組（P＜0.05）。結論加熱化療可明顯增彊阿黴素對人肝癌細胞HHCC及HepG2的增殖抑製作用，對臨床治療有藉鑒意義。
목적：탐토아매소가열화료대인간암세포HHCC급HepG2적억제작용。방법선택체외배양대수생장기HHCC급HepG2세포위연구대상，채용10%태우혈청배양액배제성세포밀도5×104/mL적단세포현액，접충우50mL적배양병중，세포분위3조，분별진행단순가열、단순화료、가열화료처리；채용MTT법검측세포증식억제솔，채용류식세포의검측세포조망솔。결과HHCC급HepG2세포증식억제솔3조간비교，차이유통계학의의（P＜0.05）；가열화료조세포증식억제솔명현고우단순가열조급단순화료조（P＜0.05）；HHCC급HepG2세포조망솔삼조간차이유통계학의의（P＜0.05），가열화료조세포조망솔명현고우단순가열조급단순화료조（P＜0.05）。결론가열화료가명현증강아매소대인간암세포HHCC급HepG2적증식억제작용，대림상치료유차감의의。
Objective To investigate the inhibiting effect of doxorubicin heating chemotherapy in proliferation of hepatic carcinoma HHCC and HepG2 cells. Methods Invitro culture HHCC and HepG2 cells in logarithmic growth phase were selected for the study,10%fetal bovine serum medium was used to formulated single cell suspension with cell density of 5×104/mL,and then inoculated in the 50mL cultivation bottle. The cells were divided into 3 groups,and given simple heating, simple chemotherapy,heating chemotherapy treatment respectively,cell proliferation was detected by MTT assay,and was apoptosis rate was detected by flow cytometer. Results Proliferation inhibition rate of HHCC and HepG2 cell among the three groups compared,the difference was statistically significant(P < 0.05), inhibition rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group (P < 0.05);apoptosis rate of HHCC and HepG2 cells of three groups compared,differences was statistically significant (P < 0.05).apoptosis rate of heating chemotherapy group was significantly higher than simple heating group and simple chemotherapy group (P<0.05). Conclusion Doxorubicin heating chemotherapy can significantly enhance inhibiting effect of doxorubicin on HHCC and HepG2 hepatoma cell, it has reference significance for clinical treatment .
