CAJ | 학술논문

目的：寻找预测MM患者GC敏感性指标。方法以MM患者作为研究对象，采用ELISA法检测MM患者血清HSP90浓度，Western Blot检测MM患者PBMNC中P23蛋白表达，探讨其与GC抵抗的关系。结果（1）MM患者血清HSP90含量明显比正常人高（P＜0.05），且GC敏感组患者较GC抵抗组患者高（P＜0.05），但各组P23血清浓度之间差异无统计学意义（P＞0.05）；（2）P23蛋白低表达患者和P23蛋白高表达患者治疗有效率分别为86%和33%，二者相比差异具有统计学意义（P＜0.05），在5年随访中，前者生存率高于后者（P＜0.05）；（3）HSP90和P23蛋白在MM1.S、MM1.R细胞株以及GC敏感和抵抗的MM患者均位于胞浆内，无定位意义。结论血清HSP90和P23蛋白表达能预测MM患者对GC的敏感性和生存情况，可用此指标指导临床用药。
목적：심조예측MM환자GC민감성지표。방법이MM환자작위연구대상，채용ELISA법검측MM환자혈청HSP90농도，Western Blot검측MM환자PBMNC중P23단백표체，탐토기여GC저항적관계。결과（1）MM환자혈청HSP90함량명현비정상인고（P＜0.05），차GC민감조환자교GC저항조환자고（P＜0.05），단각조P23혈청농도지간차이무통계학의의（P＞0.05）；（2）P23단백저표체환자화P23단백고표체환자치료유효솔분별위86%화33%，이자상비차이구유통계학의의（P＜0.05），재5년수방중，전자생존솔고우후자（P＜0.05）；（3）HSP90화P23단백재MM1.S、MM1.R세포주이급GC민감화저항적MM환자균위우포장내，무정위의의。결론혈청HSP90화P23단백표체능예측MM환자대GC적민감성화생존정황，가용차지표지도림상용약。
Objective To find the predicitive indicators of GC sensitivity in the patients with multiple myeloma (MM).MethodsThe patients with MM were selected as the study subjects. The ELISA was used to detect the serum heat-shock protein 90 (HSP90) concentraion in the patients with MM and the Western Blot was used to detect the P23 protein expression in PBMNC in the patients with MM, and their relationship with GC resistance was explored. Results (1) The patients with MM had significantly higher HSP90 content than normal people (P<0.05), and the GC sensitive group was higher than the GC resistant group (P<0.05), but the P23 serum concentration was not different between each group (P>0.05); (2) The effective treatment rates of the patients with low P23 protein expression and the patietns with high P23 protein expression were 86% and 33% respectively, with significant difference between the two groups (P<0.05), and in the 5-year follow-up visit, the former had higher survival rate than the latter (P<0.05); (3) Both HSP90 and P23 protein existed in the cytoplasm of MM1.S and MM1.R cell strains as well as the the patients with MM who were sensitive and resistant to GC, and had no locating value.Conclusion Serum HSP90 and P23 protein expression can predict the MM patients' sensitivity to GC and survival situation, thus can be used to guide clinical drug use.