CAJ | 학술논문

目的：研究山楂叶总黄酮（Total flavones of crataegus leaves ，TFCL）对大鼠肝脏缺血再灌注损伤的保护作用及机制。方法：48只健康SD大鼠随机分为6组：假手术组（Sham组）、缺血再灌注模型组（I/R组）、依达拉奉治疗组（Eda组）及TFCL高浓度（TFCL-H ，120mg/kg ，ip）、中浓度（TFCL-M ，60mg/kg ，ip）、低浓度（TFCL-L ，30mg/kg ，ip）剂量治疗组，每组8只。采用线栓法建立大鼠肝缺血再灌注模型，阻断肝门40min ，分别再灌注后50min后，治疗组于阻断肝门前30min腹腔注射依达拉奉或TFCL ，取肝组织检测超氧化物歧化酶（SOD）活性和丙二醛（MDA）含量。结果：与Sham组比较，I/R组大鼠肝组织中SOD活性明显降低，MDA含量明显增高。与I/R组比较，TFCL-H ，TFCL-M组及Eda组大鼠肝组织中SOD活性明显增高，MDA含量明显降低。I/R组与TFCL-L组对比，SOD活性及MDA含量差异无显著性，Eda组与TFCL-M相比，SOD活性和MDA含量差异无显著性。结论：腹腔注射 TFCL对大鼠肝缺血再灌注损伤具有保护作用，其机制可能与抗氧自由基作用有关。
목적：연구산사협총황동（Total flavones of crataegus leaves ，TFCL）대대서간장결혈재관주손상적보호작용급궤제。방법：48지건강SD대서수궤분위6조：가수술조（Sham조）、결혈재관주모형조（I/R조）、의체랍봉치료조（Eda조）급TFCL고농도（TFCL-H ，120mg/kg ，ip）、중농도（TFCL-M ，60mg/kg ，ip）、저농도（TFCL-L ，30mg/kg ，ip）제량치료조，매조8지。채용선전법건립대서간결혈재관주모형，조단간문40min ，분별재관주후50min후，치료조우조단간문전30min복강주사의체랍봉혹TFCL ，취간조직검측초양화물기화매（SOD）활성화병이철（MDA）함량。결과：여Sham조비교，I/R조대서간조직중SOD활성명현강저，MDA함량명현증고。여I/R조비교，TFCL-H ，TFCL-M조급Eda조대서간조직중SOD활성명현증고，MDA함량명현강저。I/R조여TFCL-L조대비，SOD활성급MDA함량차이무현저성，Eda조여TFCL-M상비，SOD활성화MDA함량차이무현저성。결론：복강주사 TFCL대대서간결혈재관주손상구유보호작용，기궤제가능여항양자유기작용유관。
Objective :To investigate the effect and mechanism of total flavones of crataegus leaves (TFCL) on hepatic ischemia/reperfusion injury of rats .Methods:48 healthy SD rats were randomly divided into 6 groups :sham operation group (Sham group) ,ischemia/reperfusion group (I/R group) ,edaravone group (Eda group) ,and TFCL-H (120mg/kg ,ip) ,TFCL-M (60mg/kg ,ip) and TFCL-L (30mg/kg ,ip) groups (n=8/group) .The hepatic ischemia/reperfusion model was produced by occlusion of hepatic portal system for 40min ,followed by 50min reperfusion .Liver tissues were taken for testing the activity of superoxide dismutase (SOD) and concentration of malondialdehyde (MDA) .Results:Compared with sham group ,the activity of SOD was significantly decreased and the concentration of MDA was signifi-cantly increased in I/R group .Compared with I/R group ,the activity of SOD were significantly increased and the con-centration of MDA were significantly decreased in TFCL-H and TFCL-M and Eda group separately .Compared with I/R group ,no differences were shown in TFCL-L group .Compared with TFCL-M group ,no differences were shown in Eda group .Conclusion:TFCL has protective effect on hepatic ischemia/reperfusion injury of rats ,which may be related to inhibition of lipid peroxidation .