CAJ | 학술논문

目的：探讨水通道蛋白（AQP）1和4在老年大鼠挫伤后肺组织中的表达。方法通过自由落体模型制作老年鼠肺挫伤模型，分别于伤后1，3和6 h处死，取右侧肺上叶组织测水含量，RT-PCR法测量AQP1和4的mRNA表达，Western印迹法检测蛋白AQP1和4的表达水平。结果伤后老年大鼠的肺组织水含量比明显增加，与假手术组相比，AQP1和4的mRNA和蛋白表达在术后逐渐升高，术后6 h有显著差异（P＜0.05）， AQP-1的蛋白表达量亦明显升高（ P＜0.05）。结论 AQP1和4在挫伤后的肺组织中表达升高，靶向调节二者的表达可能对于肺挫伤后肺水肿的治疗具有重要意义。
목적：탐토수통도단백（AQP）1화4재노년대서좌상후폐조직중적표체。방법통과자유락체모형제작노년서폐좌상모형，분별우상후1，3화6 h처사，취우측폐상협조직측수함량，RT-PCR법측량AQP1화4적mRNA표체，Western인적법검측단백AQP1화4적표체수평。결과상후노년대서적폐조직수함량비명현증가，여가수술조상비，AQP1화4적mRNA화단백표체재술후축점승고，술후6 h유현저차이（P＜0.05）， AQP-1적단백표체량역명현승고（ P＜0.05）。결론 AQP1화4재좌상후적폐조직중표체승고，파향조절이자적표체가능대우폐좌상후폐수종적치료구유중요의의。
Objective To investigate the expression of aquaporin ( AQP) 1 and 4 in lung tissues of aged rats with contusion injury . Methods The aged rat pulmonary contusion model was made by free falling body method .The rats were killed after injury for 1, 3 and 6 h. Their right upper lobe of lung tissues were took out to measure the water content .The expression of mRNA of AQP 1 and 4 were measured by PT-PCR.And the expression level of protein of AQP 1 and 4 were measured by Western blot .Results After injury, the water content in aged rats’ pulmonary tissues increased significantly .Compared with the control group , the expression of mRNA and protein of AQP 1 and 4 increased gradually after operation.And after 6 h, the differences showed statistical significance (P<0.05), as well the expression level of protein of AQP1.Conclusions The expression of AQP4 and 1 in contused lung tissue could increase significantly .Targeted adjustment might have important significance for the treatment of pulmonary edema after lung contusion .