中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
15期
4236-4238
,共3页
贺琴%李德梅%李敬会%谭华炳
賀琴%李德梅%李敬會%譚華炳
하금%리덕매%리경회%담화병
2型糖尿病%非酒精性脂肪性肝病%绞股蓝皂苷%脂质沉积
2型糖尿病%非酒精性脂肪性肝病%絞股藍皂苷%脂質沉積
2형당뇨병%비주정성지방성간병%교고람조감%지질침적
Type 2 diabetes mellitus%Nonalcoholic fatty liver disease%Lipid deposition%Gypenosides
目的：观察绞股蓝皂苷（ GPS）对2型糖尿病（ T2DM）并非酒精性脂肪性肝病（ NAFLD）大鼠肝组织脂质沉积量的影响。方法65只SPF级雄性SD大鼠，体重220～250 g，随机分为空白对照组（7只，N组），NAFLD模型组（NM 组，7只），T2DM并 NAFLD模型组（51只）。 N组给予普通饲料喂养。 NM组给予高脂饲料喂养。模型组先给予高糖高脂饲料喂养4 w；隔夜空腹腹腔注射链脲佐菌素（ STZ）40 mg/kg，继续给予高糖高脂饲料喂养至8 w，建立T2DM并NAFLD大鼠模型；将模型组分为GPS大剂量干预组（9只，JH组），予GPS 1 g· kg-1· d-1灌胃；GPS小剂量干预组（9只，JL组），予GPS 0.5 g· kg-1· d-1灌胃；T2DM并NAFLD模型组（9只，M组），同等体积纯净水灌胃；继续给予高糖高脂饲料；治疗周期6 w。实验周期14 w。检测血甘油三酯（ TG）、胆固醇（ TC）、血糖（ GLU）、肝组织TG、肝脏病理学。结果肝组织TG水平：JH组、JL组低于M组，比较有显著差异（P＜0.01）。血糖水平NM组与N组存在显著差异（P＜0.01）。血TG水平：JH组、JL组与M组有显著差异（P＜0.01）。肝脏病理学：NM 组、M 组重度NAFLD，JH组、JL组中至重度NAFLD。结论绞股蓝能够降低T2DM并NAFLD大鼠肝脏脂质沉积量。
目的：觀察絞股藍皂苷（ GPS）對2型糖尿病（ T2DM）併非酒精性脂肪性肝病（ NAFLD）大鼠肝組織脂質沉積量的影響。方法65隻SPF級雄性SD大鼠，體重220～250 g，隨機分為空白對照組（7隻，N組），NAFLD模型組（NM 組，7隻），T2DM併 NAFLD模型組（51隻）。 N組給予普通飼料餵養。 NM組給予高脂飼料餵養。模型組先給予高糖高脂飼料餵養4 w；隔夜空腹腹腔註射鏈脲佐菌素（ STZ）40 mg/kg，繼續給予高糖高脂飼料餵養至8 w，建立T2DM併NAFLD大鼠模型；將模型組分為GPS大劑量榦預組（9隻，JH組），予GPS 1 g· kg-1· d-1灌胃；GPS小劑量榦預組（9隻，JL組），予GPS 0.5 g· kg-1· d-1灌胃；T2DM併NAFLD模型組（9隻，M組），同等體積純淨水灌胃；繼續給予高糖高脂飼料；治療週期6 w。實驗週期14 w。檢測血甘油三酯（ TG）、膽固醇（ TC）、血糖（ GLU）、肝組織TG、肝髒病理學。結果肝組織TG水平：JH組、JL組低于M組，比較有顯著差異（P＜0.01）。血糖水平NM組與N組存在顯著差異（P＜0.01）。血TG水平：JH組、JL組與M組有顯著差異（P＜0.01）。肝髒病理學：NM 組、M 組重度NAFLD，JH組、JL組中至重度NAFLD。結論絞股藍能夠降低T2DM併NAFLD大鼠肝髒脂質沉積量。
목적：관찰교고람조감（ GPS）대2형당뇨병（ T2DM）병비주정성지방성간병（ NAFLD）대서간조직지질침적량적영향。방법65지SPF급웅성SD대서，체중220～250 g，수궤분위공백대조조（7지，N조），NAFLD모형조（NM 조，7지），T2DM병 NAFLD모형조（51지）。 N조급여보통사료위양。 NM조급여고지사료위양。모형조선급여고당고지사료위양4 w；격야공복복강주사련뇨좌균소（ STZ）40 mg/kg，계속급여고당고지사료위양지8 w，건립T2DM병NAFLD대서모형；장모형조분위GPS대제량간예조（9지，JH조），여GPS 1 g· kg-1· d-1관위；GPS소제량간예조（9지，JL조），여GPS 0.5 g· kg-1· d-1관위；T2DM병NAFLD모형조（9지，M조），동등체적순정수관위；계속급여고당고지사료；치료주기6 w。실험주기14 w。검측혈감유삼지（ TG）、담고순（ TC）、혈당（ GLU）、간조직TG、간장병이학。결과간조직TG수평：JH조、JL조저우M조，비교유현저차이（P＜0.01）。혈당수평NM조여N조존재현저차이（P＜0.01）。혈TG수평：JH조、JL조여M조유현저차이（P＜0.01）。간장병이학：NM 조、M 조중도NAFLD，JH조、JL조중지중도NAFLD。결론교고람능구강저T2DM병NAFLD대서간장지질침적량。
Objective To investigate the effect of Gypenosides on rat liver tissue lipid deposition with type 2 diabetes Mellitus (T2DM) and nonalcohol fatty liver disease (NAFLD) and the mechanisms.Methods 65 SPF male SD rats, body mass 220~250 g, were randomly divided into blank control group (group N, n=7), NAFLD model group (group NM, n=7), NAFLD and T2DM model group (n=51).Rats in NAFLD and T2DM model group were divided into three groups: high dose GPS group (JH, n=9) treated with 1 g· kg-1· d-1 GPS, low dose GPS group (JL, n=9) treated with 0.5 g· kg-1· d-1 GPS, model group (M, n=9) treated with the same volume of water , and high fat diet at the same time.Plasma triglycerides ( TG) and total cholesterol ( TC) , blood sugar ( BS) , TG in liver tissue and liver pathology were detected.Results TG level in group JH and group JL were lower than that of group M ( P<0.01 ).TG level in group JH was lower than that of group JL , the difference was significant ( P<0.05 ).BS level of group JH and group JL were lower than that of group M ( P<0.01 ).BS level of group JH was lower than that of group JL ( P<0.05 ).The plasma TG level of group JH and group JL were lower than that of group M ( P<0.01 ).The plasma TG level of group JH was lower than that of group JL , the difference was significant ( P<0.05 ).Liver pathology of NM group , M group developed severe fatty liver and became moderate to severe degree of NAFLD in group JH, group JL.Conclusions Gypenosides could reduce lipid deposition in the liver in rat with T 2DM and NAFLD.
