CAJ | 학술논문

目的：研究粒系集落刺激因子（ G-CSF ）和粒单系集落刺激因子（ GM-CSF ）对完全缓解急性髓系白血病（ AML）患者树突状细胞（ DC）的功能及其亚群变化。方法将36例化疗达完全缓解的AML患者根据随机数字表法分为三组，G组给予G-CSF 200μg/d皮下注射，GM组给予GM-CSF 200μg/d皮下注射，对照组（ C组）皮下注射0．9％氯化钠注射液。比较其疗效。结果 G组DC表面CD80、CD83和CD86表达均低于GM组和C组（t＝4．34、5．43、4．54、4．54、5．25、3．54，均P＜0．05），GM组DC表达CD11c高于G组和C组（t＝4．54、4．27，均P＜0．05）。 G组和GM组DC促淋巴细胞增殖能力均高于C组（t＝4．54、5．64，均P＜0.05）；GM组DC促进CD4＋T淋巴细胞增殖的能力高于G组（t＝3．54，P＜0．05）。 ELISA法检测发现，GM组DC分泌IL-12高于G组和C组；G组DC分泌IL-10高于GM组和C组（ t＝3．54、4．23、4．32、3．87，均P＜0.05）。结论 G-CSF和GM-CSF可使完全缓解AML患者DC亚群发生偏移，G-CSF促使其偏向DC2而具有一定免疫抑制作用，而GM-CSF使DC亚群偏向DC1而促进细胞免疫。
목적：연구립계집락자격인자（ G-CSF ）화립단계집락자격인자（ GM-CSF ）대완전완해급성수계백혈병（ AML）환자수돌상세포（ DC）적공능급기아군변화。방법장36례화료체완전완해적AML환자근거수궤수자표법분위삼조，G조급여G-CSF 200μg/d피하주사，GM조급여GM-CSF 200μg/d피하주사，대조조（ C조）피하주사0．9％록화납주사액。비교기료효。결과 G조DC표면CD80、CD83화CD86표체균저우GM조화C조（t＝4．34、5．43、4．54、4．54、5．25、3．54，균P＜0．05），GM조DC표체CD11c고우G조화C조（t＝4．54、4．27，균P＜0．05）。 G조화GM조DC촉림파세포증식능력균고우C조（t＝4．54、5．64，균P＜0.05）；GM조DC촉진CD4＋T림파세포증식적능력고우G조（t＝3．54，P＜0．05）。 ELISA법검측발현，GM조DC분비IL-12고우G조화C조；G조DC분비IL-10고우GM조화C조（ t＝3．54、4．23、4．32、3．87，균P＜0.05）。결론 G-CSF화GM-CSF가사완전완해AML환자DC아군발생편이，G-CSF촉사기편향DC2이구유일정면역억제작용，이GM-CSF사DC아군편향DC1이촉진세포면역。
Objective To study the granulocyte colony stimulating factor ( G-CSF ) and granulocyte macro-phage colony stimulating factor ( GM-CSF) in complete remission of acute myeloid leukemia ( AML) patients with den-dritic cells(DC)changes in the function and its subsets .Methods 36 cases of complete remission according to AML patients were randomly divided into 3 groups,G group was given G-CSF 200g/d,subcutaneous injection ,GM group were given GM-CSF 200g/d,subcutaneous injection ,the control group were injected with normal saline .The curative effects were compared.Results The expression of G in group DC,CD83 and CD86 on surface of CD80 were lower than that in GM group and C group (t=4.34,5.43,4.54,4.54,5.25,3.54,all P<0.05),GM group,DC expression of CD11c was higher than that of G group and C group (t=4.54,4.27,all P<0.05).G group and GM group DC in promoting lymphocyte proliferation were higher than those in C group (t=4.54,5.64,all P<0.05);group GM DC to promote the ability of CD4 +T lymphocyte proliferation was higher than that of G group (t=3.54,P<0.05).ELISA assay,GM group DC secretion of IL-12 than that of group G and C group;group G DC secretion of IL-10 was higher than that of GM group and C group (t=3.54.4.23,4.32,3.87,all P<0.05).Conclusion G-CSF and GM-CSF can make the complete remission in patients with AML subgroup DC shift ,the bias of G-CSF DC2 and certain immuno-suppressive effect ,while GM-CSF DC subsets of DC 1 and promotes cell immune deviation .