中国实用神经疾病杂志
中國實用神經疾病雜誌
중국실용신경질병잡지
CHINESE JOURNAL OF PRACTICAL NERVOUS DISEASES
2014年
16期
1-2,3
,共3页
吴灵芝%李水彬%成钢卫%汪华侨%孔令平
吳靈芝%李水彬%成鋼衛%汪華僑%孔令平
오령지%리수빈%성강위%왕화교%공령평
人端粒酶催化亚基(hTERT)%人胚胎大脑皮质神经元%阿尔茨海默病%神经元周期素依赖性蛋白激酶 5%cAMP反应元件结合蛋白
人耑粒酶催化亞基(hTERT)%人胚胎大腦皮質神經元%阿爾茨海默病%神經元週期素依賴性蛋白激酶 5%cAMP反應元件結閤蛋白
인단립매최화아기(hTERT)%인배태대뇌피질신경원%아이자해묵병%신경원주기소의뢰성단백격매 5%cAMP반응원건결합단백
Human telomerase reverse transcriptase(hTERT)%Human embryonic cerebral cortex neuron%Alzheimer's disease%Cyclin-dependent kinase 5(cdk5)%cAMP response element binding protein(P-CREB)
目的：对干预后人胚胎神经元周期素依赖性蛋白激酶5（cyclin-dependent kinase 5，cdk5）、cAMP反应元件结合蛋白（cAMP response element binding protein，P-CREB）表达影响。方法人胚胎大脑皮质神经元原代培养后，用 hTERT重组腺病毒转染神经元，用 Aβ25-35干预转染后神经元。实验分为转染组和非转染组。Western-blot检测 cdk5、p-CREB的变化。结果 Aβ25-35干预后，神经元内 cdk5表达量明显增加，而 hTERT基因转染组 cdk5增加的低于非转染组。干预后神经元内 p-CREB表达量明显降低，而 hTERT基因转染组，p-CREB表达量高于非转染组。结论 Aβ25-35干预人胚胎大脑皮质神经元，可以导致 cdk5的异常活化，p-CREB的降低；hTERT基因转染可有效抑制 cdk5的异常激活，防止 p-CREB的降低。
目的：對榦預後人胚胎神經元週期素依賴性蛋白激酶5（cyclin-dependent kinase 5，cdk5）、cAMP反應元件結閤蛋白（cAMP response element binding protein，P-CREB）錶達影響。方法人胚胎大腦皮質神經元原代培養後，用 hTERT重組腺病毒轉染神經元，用 Aβ25-35榦預轉染後神經元。實驗分為轉染組和非轉染組。Western-blot檢測 cdk5、p-CREB的變化。結果 Aβ25-35榦預後，神經元內 cdk5錶達量明顯增加，而 hTERT基因轉染組 cdk5增加的低于非轉染組。榦預後神經元內 p-CREB錶達量明顯降低，而 hTERT基因轉染組，p-CREB錶達量高于非轉染組。結論 Aβ25-35榦預人胚胎大腦皮質神經元，可以導緻 cdk5的異常活化，p-CREB的降低；hTERT基因轉染可有效抑製 cdk5的異常激活，防止 p-CREB的降低。
목적：대간예후인배태신경원주기소의뢰성단백격매5（cyclin-dependent kinase 5，cdk5）、cAMP반응원건결합단백（cAMP response element binding protein，P-CREB）표체영향。방법인배태대뇌피질신경원원대배양후，용 hTERT중조선병독전염신경원，용 Aβ25-35간예전염후신경원。실험분위전염조화비전염조。Western-blot검측 cdk5、p-CREB적변화。결과 Aβ25-35간예후，신경원내 cdk5표체량명현증가，이 hTERT기인전염조 cdk5증가적저우비전염조。간예후신경원내 p-CREB표체량명현강저，이 hTERT기인전염조，p-CREB표체량고우비전염조。결론 Aβ25-35간예인배태대뇌피질신경원，가이도치 cdk5적이상활화，p-CREB적강저；hTERT기인전염가유효억제 cdk5적이상격활，방지 p-CREB적강저。
Objective To observe the effects of hTERT on cdk5,P-CREB and P16 of human embryonic cerebral cortex neuron after Aβinvention.Methods Primary cerebral cortex neurons of human embryo cultured were transfected with hTERT. The neurons were dealt with Aβ25-35.Two groups were divided into the transfected group and the non-transfected group.The expressions of cdk5,p-CREB were measured by Western-blot.Results The expression of cdk5 was obviously up-regulated af-ter the intervention of Aβ25-35,but in the group transfected with pSU-CMV-hTERT,the expression of cdk5 was decreased than that in non-transfected group.The expression of p-CREB was obviously down-regulated after the intervention of Aβ25-35, but in the group transfected with pSU-CMV-hTERT,the express of p-CREB was higher than that in the non-transfected group.Conclusion Intervention on human embryonic cerebral cortex neuron with Aβmay lead to abnormal activation of cdk5 and decrease of p-CREB.However,the hTERT transfection could inhibit abnormal activation of cdk5 and the decrease of p-CREB.
