中外医学研究
中外醫學研究
중외의학연구
CHINESE AND FOREIGN MEDICAL RESEARCH
2014年
20期
3-5
,共3页
壳聚糖%磷酸三钙%支架%组织工程%重结晶法
殼聚糖%燐痠三鈣%支架%組織工程%重結晶法
각취당%린산삼개%지가%조직공정%중결정법
Chitosan%Tricalcium phosphate%Scaffold%Tissue engineering%Recrystallization
目的：研究壳聚糖-磷酸三钙复合材料(CTCP)支架的制备新方法及其制品性能。方法：采用重结晶-冻干法制备支架，通过大体观察、pH值、孔隙率及孔隙直径测定、细胞复合培养来研究支架性能，直接冻干法支架作为对照。结果：重结晶法最优组较对照组在孔隙率上比较差异有统计学意义(P<0.05)。孔隙直径也更理想，以100~300μm为主；细胞能进入材料的孔隙并生长良好。结论：重结晶法制备聚糖-磷酸三钙支架有更好的孔隙质量；较高的重结晶温度和速率可能是提高孔隙质量的有利因素。
目的：研究殼聚糖-燐痠三鈣複閤材料(CTCP)支架的製備新方法及其製品性能。方法：採用重結晶-凍榦法製備支架，通過大體觀察、pH值、孔隙率及孔隙直徑測定、細胞複閤培養來研究支架性能，直接凍榦法支架作為對照。結果：重結晶法最優組較對照組在孔隙率上比較差異有統計學意義(P<0.05)。孔隙直徑也更理想，以100~300μm為主；細胞能進入材料的孔隙併生長良好。結論：重結晶法製備聚糖-燐痠三鈣支架有更好的孔隙質量；較高的重結晶溫度和速率可能是提高孔隙質量的有利因素。
목적：연구각취당-린산삼개복합재료(CTCP)지가적제비신방법급기제품성능。방법：채용중결정-동간법제비지가，통과대체관찰、pH치、공극솔급공극직경측정、세포복합배양래연구지가성능，직접동간법지가작위대조。결과：중결정법최우조교대조조재공극솔상비교차이유통계학의의(P<0.05)。공극직경야경이상，이100~300μm위주；세포능진입재료적공극병생장량호。결론：중결정법제비취당-린산삼개지가유경호적공극질량；교고적중결정온도화속솔가능시제고공극질량적유리인소。
Objective:To explore the new method of chitosan/tricalcium phosphate(CTCP) scaffold preparation,and the scaffold’s performance assessment. Method:CTCP scaffolds were prepared by recrystallization method and ordinary mode,which were investigated by macroscopic observation,pH value,porosity, pore diameter and cell co-culture.Result:The best experimental group present significantly difference between control group in porosity(P<0.05).Which had better pore diameter in 100-300μm mainly and cell co-cultured present well-growing through the pore.Conclusion:CTCP scaffold has better pore quality,higher recrystal temperature and it’s rate could be the favorable factor to improve the quality of scaffold pore.
