CAJ | 학술논문

研究病毒感染对白斑综合症病毒基因的录调控的影响，将Caspase调控区的 DNA进行生物素标记，然后与链霉亲和素修饰的琼脂糖结合。通过下拉发现，Caspase基因的启动子序列与 white spot syndromic vi-rus（WSSV）病毒的两个蛋白 Vp38和 Vp41B有相互作用。通过荧光素酶报告基因发现，Vp38和 Vp41B对Caspase启动子活性分别有抑制和激活作用。采用RNAi技术下调两个WSSV蛋白的表达后，研究发现：Vp38和 Vp41 B分别对对虾血细胞的凋亡水平有促进和抑制的调控作用。
연구병독감염대백반종합증병독기인적록조공적영향，장Caspase조공구적 DNA진행생물소표기，연후여련매친화소수식적경지당결합。통과하랍발현，Caspase기인적계동자서렬여 white spot syndromic vi-rus（WSSV）병독적량개단백 Vp38화 Vp41B유상호작용。통과형광소매보고기인발현，Vp38화 Vp41B대Caspase계동자활성분별유억제화격활작용。채용RNAi기술하조량개WSSV단백적표체후，연구발현：Vp38화 Vp41 B분별대대하혈세포적조망수평유촉진화억제적조공작용。
The prawn Caspase gene was previously found to play important roles in protecting host from viral infection. In order to further delineate the transcriptional regulation of Caspase in response to viral infection,the Caspase promoter was biotin?labeled and bind to streptavidin?beads.Through pulldown assay the Caspase promoter was founded to interact with two white spot syndromic virus (WSSV)proteins Vp38 and Vp41B.Luciferase reporter assay revealed that Vp38 and Vp41B act as repressor and activator of Caspase transcription respectively.RNAi inhibition of Vp38 and Vp41B indi-cate the two WSSV proteins play roles on the regulation of prawn hymocyte apoptosis.These results provided insights in-to the immune mechanism of invertebrates,and will also be helpful for shrimp viral disease control.