CAJ | 학술논문

[目的]建立操作简便、软件依赖性低、结果易于交流的脉冲场凝胶电泳（ PFGE）指纹图谱分析新方法。[方法]按PulseNet标准操作规程，对44株副溶血性弧菌进行PFGE分型试验，分别利用分区条带计数法和BioN-umerics软件对图谱进行分析，并以型别数、成簇数和Simpson’ s多样性指数（ DI）对2种分析方法进行比较。[结果]分区条带计数法可将44株菌分为32个型（DI＝97．6％），能发现5簇。 BioNumerics 聚类分析可分为29个BN型（DI＝95．5％），能发现4簇。在发现菌株成簇的能力上，2种分析方法的差异无统计学意义。[结论]条带计数法能大大减少肉眼观察的图谱数量，具有操作简便、成本低、对软件依赖性小、结果显示简单直接的特点，能直观反映菌株条带的分布特征，因此应能满足分型信息共享与交流的要求。
[목적]건립조작간편、연건의뢰성저、결과역우교류적맥충장응효전영（ PFGE）지문도보분석신방법。[방법]안PulseNet표준조작규정，대44주부용혈성호균진행PFGE분형시험，분별이용분구조대계수법화BioN-umerics연건대도보진행분석，병이형별수、성족수화Simpson’ s다양성지수（ DI）대2충분석방법진행비교。[결과]분구조대계수법가장44주균분위32개형（DI＝97．6％），능발현5족。 BioNumerics 취류분석가분위29개BN형（DI＝95．5％），능발현4족。재발현균주성족적능력상，2충분석방법적차이무통계학의의。[결론]조대계수법능대대감소육안관찰적도보수량，구유조작간편、성본저、대연건의뢰성소、결과현시간단직접적특점，능직관반영균주조대적분포특정，인차응능만족분형신식공향여교류적요구。
To establish a new method for PFGE fingerprint analysis , which is easy-to-operate , inexpensive , software-low-dependent and readily-exchangeable . [ Methods ] A group of 44 vibrio parahaemolyticus was subtyped according to PulseNet standardized PFGE protocol .The fingerprint was analyzed with new gel-partition bands counting method and BioNumerics clustering separately and then the results by the two methods were compared with type number , cluster number and Simpson ’ s Index of Diversity ( DI) . [ Results] The 44 isolates could be typed into 32 types and 5 clusters were found by gel-partition bands counting method ( DI=97.6%) .BioNumerics could type the same isolates into 29 types and 4 clusters were found ( DI=95.5%) .The difference in the ability of finding clusters between the two methods was not statistically significant . [ Conclusion] Gel-partition bands counting method is based on the bands distribution among finger print and greatly reduces the number of visually observed spectrum , which is easy-to-operate, inexpensive , software-low-dependent and readily-exchangeable .