临床肺科杂志
臨床肺科雜誌
림상폐과잡지
JOUNAL OF CLINICAL PULMONARY MEDICINE
2014年
11期
1951-1952,1953
,共3页
苏斌涛%崔天盆%罗光伟%杨硕
囌斌濤%崔天盆%囉光偉%楊碩
소빈도%최천분%라광위%양석
慢性阻塞性肺疾病%血管内皮生长因子%细胞凋亡
慢性阻塞性肺疾病%血管內皮生長因子%細胞凋亡
만성조새성폐질병%혈관내피생장인자%세포조망
chronic obstructive pulmonary disease%vascular endothelial growth factor%cell apoptosis
目的:探讨慢性阻塞性肺疾病患者( COPD)血清中,血管内皮生长因子( VEGF)的表达及细胞凋亡的变化,研究两者间的相关性。方法检测62例COPD稳定期患者、46例健康体检者血清中VEGF的表达,并采用流式细胞术检测体外培养的外周血单核细胞0,2,24h的凋亡率。结果 COPD稳定期患者体内血清VEGF表达水平明显高于对照组,P<0.05。单核细胞体外培养2,24 h凋亡率显著低于对照组,P<0.05。且VEGF的表达与外周血单核细胞的凋亡呈负相关(P<0.05)。结论 VEGF在COPD患者细胞凋亡中扮演重要作用。
目的:探討慢性阻塞性肺疾病患者( COPD)血清中,血管內皮生長因子( VEGF)的錶達及細胞凋亡的變化,研究兩者間的相關性。方法檢測62例COPD穩定期患者、46例健康體檢者血清中VEGF的錶達,併採用流式細胞術檢測體外培養的外週血單覈細胞0,2,24h的凋亡率。結果 COPD穩定期患者體內血清VEGF錶達水平明顯高于對照組,P<0.05。單覈細胞體外培養2,24 h凋亡率顯著低于對照組,P<0.05。且VEGF的錶達與外週血單覈細胞的凋亡呈負相關(P<0.05)。結論 VEGF在COPD患者細胞凋亡中扮縯重要作用。
목적:탐토만성조새성폐질병환자( COPD)혈청중,혈관내피생장인자( VEGF)적표체급세포조망적변화,연구량자간적상관성。방법검측62례COPD은정기환자、46례건강체검자혈청중VEGF적표체,병채용류식세포술검측체외배양적외주혈단핵세포0,2,24h적조망솔。결과 COPD은정기환자체내혈청VEGF표체수평명현고우대조조,P<0.05。단핵세포체외배양2,24 h조망솔현저저우대조조,P<0.05。차VEGF적표체여외주혈단핵세포적조망정부상관(P<0.05)。결론 VEGF재COPD환자세포조망중분연중요작용。
Objective To investigate the serum level of VEGF in COPD patients and its correlation with cell apoptosis. Methods The level of VEGF was measured from 62 COPD patients and 46 healthy persons. Flow cytom-etry was used to detect mononuclear cell apoptosis. Results The expression of VEGF in the COPD group was signifi-cantly higher than that in the healthy control group (P<0. 05). 2 and 24 hours after culture, the percentage of apop-totic mononuclear cells in the COPD group was significantly lower than that in the control group (P<0. 05). Serum VEGF expression was negatively correlated with peripheral blood mononuclear cell apoptosis ( r = -0. 35, P <0. 05). Conclusion VEGF plays an important role in the cell apoptosis from pations with COPD.