CAJ | 학술논문

目的：建立板蓝根颗粒清洁验证的HPLC法。方法：色谱柱为C18柱；流动相为甲醇：0.02%磷酸水溶液（7∶93）；检测波长245nm；流速0.8ml/min；柱温30℃；进样量10μL。理论板数按R，S-表告依春峰计应不得低于5000。结果：板蓝根颗粒中表告依春的含量在3.9～95μg/ml （A=85322C-72006，r=0.9995）内呈良好线性关系，平均回收率102.80%，RSD 1.22%；表告依春的残留量在0.04～1.0μg/ml（A=90115C-13204，r=0.9997）内呈良好线性关系。结论：方法简便，稳定可靠，可以用于板蓝根颗粒中表告依春的质量控制；清洁后水样中表告依春的残留量低于限度。
목적：건립판람근과립청길험증적HPLC법。방법：색보주위C18주；류동상위갑순：0.02%린산수용액（7∶93）；검측파장245nm；류속0.8ml/min；주온30℃；진양량10μL。이론판수안R，S-표고의춘봉계응불득저우5000。결과：판람근과립중표고의춘적함량재3.9～95μg/ml （A=85322C-72006，r=0.9995）내정량호선성관계，평균회수솔102.80%，RSD 1.22%；표고의춘적잔류량재0.04～1.0μg/ml（A=90115C-13204，r=0.9997）내정량호선성관계。결론：방법간편，은정가고，가이용우판람근과립중표고의춘적질량공제；청길후수양중표고의춘적잔류량저우한도。
Objective:To estabLish an HPLC method of BanLangen GranuLes’ cLeaning vaLidation.Method:The sampLes were used with C18 and the mobiLe phase of methanoL-0.02% phosphate (7∶93).The fLow rate was set at 0.8ml/min and the detection waveLength was set at 245nm.CoLumn temperature was 30℃ and sampLe voLume was 10uL.TheoreticaL pate number shaLL not be than 5000.ResuLts:The Linear range was3.9~95μg/ml (A=85322C-72006 r=0.9995),and the average recovery was 102.8% with the RSD was 1.22%.The residuaL of epigoitrin from 0.04 to 1.0μg/ml (A=90115C-1320.4 r=0.9997) in Linear.ConcLusion :This method is convenient, repeatabLe and reLiabLe, and couLd be used for the quantitative controL of BanLangen granuLes; After cLeaning the residues in the water was Lesser than epigotrin by Limit residues.