CAJ | 학술논문

目的：建立苦豆草药材中生物碱含量的测定方法。方法：采用HPLC法测定，色谱柱为Agilent TC-C18（2）（250mm×4.6mm，5μm）；流动相为乙腈-0.05mol·L-1磷酸二氢钾水溶液（三乙胺2.0ml/L），梯度洗脱；流速为1.0ml/min，柱温30℃；检测波长205nm。结果：苦豆草中主要生物碱槐定碱在0.00804mg·ml-1～0.2412mg·ml-1范围内有良好的线性关系，回收率为99.3%，RSD为1.63%；氧化苦参碱在0.00808mg·ml-1～0.2424mg·ml-1范围内有良好的线性关系，回收率为99.4%，RSD为1.82%。结论：该方法简便易行、结果准确、可靠，重复性好，可用于苦豆草中生物碱的定量分析方法。
목적：건립고두초약재중생물감함량적측정방법。방법：채용HPLC법측정，색보주위Agilent TC-C18（2）（250mm×4.6mm，5μm）；류동상위을정-0.05mol·L-1린산이경갑수용액（삼을알2.0ml/L），제도세탈；류속위1.0ml/min，주온30℃；검측파장205nm。결과：고두초중주요생물감괴정감재0.00804mg·ml-1～0.2412mg·ml-1범위내유량호적선성관계，회수솔위99.3%，RSD위1.63%；양화고삼감재0.00808mg·ml-1～0.2424mg·ml-1범위내유량호적선성관계，회수솔위99.4%，RSD위1.82%。결론：해방법간편역행、결과준학、가고，중복성호，가용우고두초중생물감적정량분석방법。
Objective Establish a method Sophora medicinal alkaloid content. Methods by HPLC column was Agilent Zorbax SB-C 18 column (250mm ×4.6mm,5μm);mobile phase of acetonitrile-0.05mol·L-1 potassium dihydrogen phosphate (triethylamine 2.0ml/L), gradient elution off;flow 1.0ml/min, column temperature was 30℃;detection wavelength of 205nm. Results The main alkaloid sophoridine a good linear relationship ·ml-1~0.2412mg·ml-1 range of 0.00804mg, recovery was 99.3%, RSD was 1.63%; Oxymatrine at 0.00808mg· ml-1~0.2424mg·ml-1 has a good linear relationship within the range of recovery was 99.4%, RSD was 1.82%. Conclusion The method is simple, accurate, reproducible and can be used for quantitative analysis of alkaloid methods.