CAJ | 학술논문

目的：研究胸水多克隆抗卡介苗( BCG) IgM抗体的浓度是否有助于结核性胸膜炎的诊断。方法序贯纳入胸腔积液患者102例，进入研究的患者于第一次胸穿时留取胸水待检，以胸膜活检病理检查为金标准，综合其他临床资料，剔除诊断未明者，分为结核性胸膜炎组( n=40)及对照组( n=24)。建立以BCG全菌细胞包被作为固相载体的间接法ELISA反应体系，检测胸水多克隆抗BCG抗体的浓度。评价其在结核性胸膜炎诊断方面的效能。结果病例组胸水抗BCGIgM抗体浓度显著高于对照组( P<0．001)。以对照组抗体水平平均值加2倍标准差为诊断界值，阳性预测值可高达96．0%。结论胸水多克隆抗卡介苗( BCG) IgM抗体的浓度对结核性胸膜炎的诊断有一定价值。
목적：연구흉수다극륭항잡개묘( BCG) IgM항체적농도시부유조우결핵성흉막염적진단。방법서관납입흉강적액환자102례，진입연구적환자우제일차흉천시류취흉수대검，이흉막활검병리검사위금표준，종합기타림상자료，척제진단미명자，분위결핵성흉막염조( n=40)급대조조( n=24)。건립이BCG전균세포포피작위고상재체적간접법ELISA반응체계，검측흉수다극륭항BCG항체적농도。평개기재결핵성흉막염진단방면적효능。결과병례조흉수항BCGIgM항체농도현저고우대조조( P<0．001)。이대조조항체수평평균치가2배표준차위진단계치，양성예측치가고체96．0%。결론흉수다극륭항잡개묘( BCG) IgM항체적농도대결핵성흉막염적진단유일정개치。
Objective To evaluate the diagnostic value of the levels of anti-BCG IgM antibodies in tubercu-lous pleurisy. Methods 102 patients were consequently included in this research, and the pleural effusion samples were collected from each subject in the first thoracocentesis procedure. Individuals historically diagnosed as having tu-berculosis were defined as tuberculous pleurisy (40 cases). Subjects clinically diagnosed with other well defined dis-eased were included in the control group (24 cases). We developed an indirect whole bacterial cell ELISA test to de-tect polycolonal anti-BCG IgM antibodies of pleural effusion in the tuberculous pleurisy group and the control group. And then ROC analysis was performed to evaluate the value of the test for the diagnosis of tuberculous pleurisy. Re-sults Compared with the control group, the levels of polycolonal anti-BCG IgM antibodies of pleural effusion in the tuberculous pleurisy group statically significantly increased (P<0. 001). The average of antibody levels in the con-trol group plus 2SD as diagnostic cutoff point was appropriate. Conclusion Pleural effusion polycolonal anti-BCG IgM antibodies can be proposed as a diagnostic marker of tuberculous pleurisy.