CAJ | 학술논문

目的：研究耐药肺结核患者支气管灌洗液CD4+CD2+5 Treg细胞水平与非耐药肺结核患者之间的差异。方法采用流式细胞技术测定40例耐药肺结核患者、60例非耐药肺结核患者及50例健康体检者外周血及支气管肺泡灌洗液CD4+CD2+5 Treg细胞水平，观察三组之间的差别。结果耐药组外周血CD4+CD2+5 Treg细胞水平(15．50±1．49)%，非耐药组(11.62±2．34)%，对照组(6．74±2．13)%，三组间有统计学差异(P<0．05)；耐药组支气管肺泡灌洗液CD4+CD2+5 Treg细胞水平(19．33±2．15)%，非耐药组(15．56±1．98)%，两组间有统计学差异(P<0．05)。结论调节性T细胞在耐药肺结核局部病灶中发挥重要的免疫抑制作用，它与肺结核耐药机制的形成可能有关。
목적：연구내약폐결핵환자지기관관세액CD4+CD2+5 Treg세포수평여비내약폐결핵환자지간적차이。방법채용류식세포기술측정40례내약폐결핵환자、60례비내약폐결핵환자급50례건강체검자외주혈급지기관폐포관세액CD4+CD2+5 Treg세포수평，관찰삼조지간적차별。결과내약조외주혈CD4+CD2+5 Treg세포수평(15．50±1．49)%，비내약조(11.62±2．34)%，대조조(6．74±2．13)%，삼조간유통계학차이(P<0．05)；내약조지기관폐포관세액CD4+CD2+5 Treg세포수평(19．33±2．15)%，비내약조(15．56±1．98)%，량조간유통계학차이(P<0．05)。결론조절성T세포재내약폐결핵국부병조중발휘중요적면역억제작용，타여폐결핵내약궤제적형성가능유관。
Objective To study the differences in CD4+CD2+5 Treg in bronchoalveolar lavage fluid between drug-resistant tuberculosis patients and non-drug-resistant tuberculosis patients. Methods Flow cytometry was ap-plied to detect the expression percentage of CD4+CD2+5 Treg in peripheral blood and bronchoalveolar lavage fluid of drug-resistant tuberculosis patients (n=40), non-drug-resistant tuberculosis patients (n=40), and healthy people ( n=50 ) . The differences were observed and compared among the three groups. Results The percentage of CD4+CD2+5 Treg of peripheral blood in the drug-resistant group accounted for (15. 50 ± 1. 49)%, (11. 62 ± 2. 34)%in the non-drug-resistant group, and (6. 74 ± 2. 13)% in the control group, with statistical differences among the three groups (P<0.05). The percentage of CD4+CD2+5Treg of bronchoalveolar lavage fluid in the drug-resistant group accounted for (19. 33 ± 2. 15)% and (15. 56 ± 1. 98)% in the non-drug-resistant group (P<0. 05). Conclusion Treg cells play an important role in the immunosuppressive response at the local disease sites of patients with drug-re-sistant tuberculosis. It may be involved in the formation of the mechanism of drug-resistant.