中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
18期
54-55
,共2页
高效液相色谱法%妇炎舒胶囊%马钱苷%含量测定
高效液相色譜法%婦炎舒膠囊%馬錢苷%含量測定
고효액상색보법%부염서효낭%마전감%함량측정
high performance liquid chromatography%Fuyanshu Capsules%loganin%content determination
目的：建立测定妇炎舒胶囊中马钱苷含量的高效液相色谱法。方法采用 Agela Venusil MP C18色谱柱（250 mm ×4.6 mm，5μm），流动相为甲醇-磷酸盐缓冲溶液（23：77），流速为1.0 mL / min，检测波长为236 nm，柱温为30℃。结果马钱苷进样量在0.0544~0.8704μg（ r =0.9999）范围内与峰面积积分值呈良好线性关系，平均加样回收率为98.45%，RSD 为0.89%（ n =9）。结论该方法简便、灵敏、准确、可靠，可用于妇炎舒胶囊中马钱苷的含量测定。
目的：建立測定婦炎舒膠囊中馬錢苷含量的高效液相色譜法。方法採用 Agela Venusil MP C18色譜柱（250 mm ×4.6 mm，5μm），流動相為甲醇-燐痠鹽緩遲溶液（23：77），流速為1.0 mL / min，檢測波長為236 nm，柱溫為30℃。結果馬錢苷進樣量在0.0544~0.8704μg（ r =0.9999）範圍內與峰麵積積分值呈良好線性關繫，平均加樣迴收率為98.45%，RSD 為0.89%（ n =9）。結論該方法簡便、靈敏、準確、可靠，可用于婦炎舒膠囊中馬錢苷的含量測定。
목적：건립측정부염서효낭중마전감함량적고효액상색보법。방법채용 Agela Venusil MP C18색보주（250 mm ×4.6 mm，5μm），류동상위갑순-린산염완충용액（23：77），류속위1.0 mL / min，검측파장위236 nm，주온위30℃。결과마전감진양량재0.0544~0.8704μg（ r =0.9999）범위내여봉면적적분치정량호선성관계，평균가양회수솔위98.45%，RSD 위0.89%（ n =9）。결론해방법간편、령민、준학、가고，가용우부염서효낭중마전감적함량측정。
Objective To establish a high performance liquid chromatography(HPLC) method for the content determination of loganin in Fuyanshu Capsules. Methods The Agela Venusil MP C18 column(250 mm × 4. 6 mm,5 μm) was adopted with methanol - phosphate buffer solution(23 : 77)as the mobile phase,the flow rate was 1. 0 mL / min,the detection wavelength was 236 nm and the column temperature was 30 ℃ . Results The sample size of loganin in the range of 0. 054 4 - 0. 870 4 μg( r = 0. 999 9)showed good linear relationship with the peak area,the average recovery rate was 98. 45% ,RSD was 0. 89%( n = 9). Conclusion This method is simple, sensitive,accurate and reliable,which can be used for the quality control of Fuyanshu Capsules.
