中国药业
中國藥業
중국약업
CHINA PHARMACEUTICALS
2014年
18期
46-47
,共2页
癫痫清颗粒%高效液相色谱法%芒果苷%芍药苷%二苯乙烯苷
癲癇清顆粒%高效液相色譜法%芒果苷%芍藥苷%二苯乙烯苷
전간청과립%고효액상색보법%망과감%작약감%이분을희감
Dianxianqing Granules%high performance liquid chromatogrephy%mangiferin%paeoniflorin%stilbeneglycoside
目的：建立同时测定癫痫清颗粒中芒果苷、芍药苷、二苯乙烯苷含量的高效液相色谱（HPLC）法。方法采用 HPLC 波长切换法同时对3组分含量进行测定，色谱柱为 Kromasil C18柱（250 mm ×4.6 mm，5μm），以乙腈和0.2%醋酸为流动相，梯度洗脱，用二极管阵列检测器检测，检测波长为258，230，320 nm。结果芒果苷、芍药苷、二苯乙烯苷在进样量分别为0.152~1.52，0.480~4.80，0.248~2.48μg 范围内与峰面积呈良好线性关系，加样回收率分别为101.00%，102.38%，99.94%，RSD 分别为2.86%，2.38%，2.17%( n =9)。结论该研究为癫痫清颗粒的多组分定量测定和质量控制提供了一种简单、可靠的方法。
目的：建立同時測定癲癇清顆粒中芒果苷、芍藥苷、二苯乙烯苷含量的高效液相色譜（HPLC）法。方法採用 HPLC 波長切換法同時對3組分含量進行測定，色譜柱為 Kromasil C18柱（250 mm ×4.6 mm，5μm），以乙腈和0.2%醋痠為流動相，梯度洗脫，用二極管陣列檢測器檢測，檢測波長為258，230，320 nm。結果芒果苷、芍藥苷、二苯乙烯苷在進樣量分彆為0.152~1.52，0.480~4.80，0.248~2.48μg 範圍內與峰麵積呈良好線性關繫，加樣迴收率分彆為101.00%，102.38%，99.94%，RSD 分彆為2.86%，2.38%，2.17%( n =9)。結論該研究為癲癇清顆粒的多組分定量測定和質量控製提供瞭一種簡單、可靠的方法。
목적：건립동시측정전간청과립중망과감、작약감、이분을희감함량적고효액상색보（HPLC）법。방법채용 HPLC 파장절환법동시대3조분함량진행측정，색보주위 Kromasil C18주（250 mm ×4.6 mm，5μm），이을정화0.2%작산위류동상，제도세탈，용이겁관진렬검측기검측，검측파장위258，230，320 nm。결과망과감、작약감、이분을희감재진양량분별위0.152~1.52，0.480~4.80，0.248~2.48μg 범위내여봉면적정량호선성관계，가양회수솔분별위101.00%，102.38%，99.94%，RSD 분별위2.86%，2.38%，2.17%( n =9)。결론해연구위전간청과립적다조분정량측정화질량공제제공료일충간단、가고적방법。
Objective To establish a HPLC method for simultaneous determination of mangiferin,paeoniflorin and stilbeneglycoside in Di-anxianqing Granules by high performance liquid chromatogrephy(HPLC). Methods The HPLC wavelength switching method was adopted to determine the contents of these three constituents. The Kromasil C18 column(200 mm × 4. 6 mm,5 μm ) was adopted,the mobile phase consisted of acetonitrile - 0. 2% acetic acid aqueous solution for conducting the gradient elution. The detection was conducted by using the diode array detector(DAD). The wavelength of UV detector was set at 258,230,320 nm. Results Mangiferin,paeoniflorin and stilbeneglycoside had good linearity in the ranges of 0. 152 - 1. 52,0. 480 - 4. 80,0. 248 - 2. 48 μg,respectively. The average recovery rates of the three constituents were 101. 00% ,102. 38% ,99. 94% ,respectively and RSD were 2. 86% ,2. 38% ,2. 17% ( n = 9),respectively. Conclusion This method is simple and reliable,which can be used for the quantitative determination of multiple constituents and the quality control of Dianxianqing Granules.
