CAJ | 학술논문

目的：提高并完善阿归养血颗粒的质量标准。方法采用薄层色谱（TLC）法对白芍、黄芪、甘草进行鉴别，采用高效液相色谱（HPLC）法对当归和川芎进行鉴别；同时采用 HPLC 法测定芍药苷的含量，色谱柱为 Agilent TC - C18柱（250 mm ×4.6 mm，5μm），流动相为乙腈-水（10：90），流速为1.0 mL / min，检测波长为230 nm。结果 TLC 法色谱斑点清晰，专属性强，重复性良好。芍药苷对照品进样量在0.04856~0.9712μg（ r =0.9999）范围内与峰面积呈良好线性关系，平均回收率为99.47%，RSD 为1.23%（ n =6）。结论该方法简便、可靠、准确，可用于该制剂的质量控制。
목적：제고병완선아귀양혈과립적질량표준。방법채용박층색보（TLC）법대백작、황기、감초진행감별，채용고효액상색보（HPLC）법대당귀화천궁진행감별；동시채용 HPLC 법측정작약감적함량，색보주위 Agilent TC - C18주（250 mm ×4.6 mm，5μm），류동상위을정-수（10：90），류속위1.0 mL / min，검측파장위230 nm。결과 TLC 법색보반점청석，전속성강，중복성량호。작약감대조품진양량재0.04856~0.9712μg（ r =0.9999）범위내여봉면적정량호선성관계，평균회수솔위99.47%，RSD 위1.23%（ n =6）。결론해방법간편、가고、준학，가용우해제제적질량공제。
Objective To improve and perfect the quality standard for Egui Yangxue Granules. Methods Radix angelicae sinensis and Rhizoma chuanxiong were identified by HPLC. Radix paeoniae alba,glycyrrhiza uralensis and Radix astragali were indentified by TLC. The content of paeoniflorin in Radix paeoniae alba was determined by HPLC. The HPLC system consisted of the Agilent TC - C18 column(250 mm × 4. 6 mm,5 μm),with acetonitrile - water(10 : 90)as the mobile phase,the flow rate of 1. 0 mL / min and the detection wavelength at 230 nm. Results The developed TLC spots were quite clear,and the TLC method was simple with strong specificity and good reproducibility. The linear range of paeoniflorin was 0. 048 56 - 0. 971 2 μg( r = 0. 999 9). The average recovery rate was 99. 47% , RSD was 1. 23%( n = 6). Conclusion The established method is simple,reliable,accurate and suitable for the quality control of Egui Yangxue Granules.