CAJ | 학술논문

目的：比较不同产地与规格半夏中4种核苷（或碱基）的含量。方法测定甘肃省及省外（贵州、江西等）13个半夏药材样品中4种核苷（或碱基）的含量，并进行样品聚类分析，发现药材的道地性；同时选取其中3个产地（甘肃清水永靖、贵州、云南）的3种规格（大、中、小）半夏进行核苷（或碱基）含量测定，比较含量差异。采用高效液相色谱法同时测定4种核苷（或碱基）的含量，并用 SPSS 统计软件进行系统聚类分析和嵌套设计资料方差分析。结果系统聚类分析，甘肃省半夏聚为一类，省外半夏聚为另一类；嵌套设计资料方差分析， P =0.749>0.01，不同规格样品间含量差异无统计学意义。结论半夏药材的核苷（或碱基）含量跟产地有关，甘肃省与省外可很好地区分，说明了甘肃半夏的道地性特征；但不同规格间样品核苷（或碱基）含量无明显差异，说明规格不能作为评判半夏药材质量好坏的指标。
목적：비교불동산지여규격반하중4충핵감（혹감기）적함량。방법측정감숙성급성외（귀주、강서등）13개반하약재양품중4충핵감（혹감기）적함량，병진행양품취류분석，발현약재적도지성；동시선취기중3개산지（감숙청수영정、귀주、운남）적3충규격（대、중、소）반하진행핵감（혹감기）함량측정，비교함량차이。채용고효액상색보법동시측정4충핵감（혹감기）적함량，병용 SPSS 통계연건진행계통취류분석화감투설계자료방차분석。결과계통취류분석，감숙성반하취위일류，성외반하취위령일류；감투설계자료방차분석， P =0.749>0.01，불동규격양품간함량차이무통계학의의。결론반하약재적핵감（혹감기）함량근산지유관，감숙성여성외가흔호지구분，설명료감숙반하적도지성특정；단불동규격간양품핵감（혹감기）함량무명현차이，설명규격불능작위평판반하약재질량호배적지표。
Objective To compare the contents of four nucleosides or bases in different habitats and specifications of Rhizoma Pinellia ternate. Methods The HPLC method was adopted to simultaneously determine the contents of four nucleosides or bases in 13 samples of Rhizoma Pinellia ternate medicinal material from Gansu province and outside the province(Guizhou,Jiangxi,etc. ). The hierarchical -cluster analysis was performed for finding the genuineness of medicinal material;at the same time the samples of Rhizoma Pinellia ter-nate were selected from 3 kinds of specification(large,middle small)in 3 habitats(Gansu Qingshui Yongjing,Guizhou,Yunnan)for de-tecting the nucleosides or bases content. The SPSS statistical software was used to perform the hierarchical - cluster analysis and nested ANOVA analysis. Results The hierarchical - cluster analysis showed that Rhizoma Pinellia ternate from Gansu province clustered into one class and those from other provinces clustered into another class. In the nested ANOVA analysis,P = 0. 749 > 0. 01,which showed that the difference in content among different specifications had no statistical significance. Conclusion The nucleoside or bases content of Rhizoma Pinellia ternate is related with the habitat of the medicinal material,and Rhizoma Pinellia ternate from Gansu has a good distinction with others,which indicating the Gansu Pinellia's genuineness characteristics;the nucleoside or bases content in different specifications of Rhizoma Pinellia ternate has no obvious difference,which indicating that the specification can not serve as an index for judging the quality of Rhizoma Pinellia ternate.