国际检验医学杂志
國際檢驗醫學雜誌
국제검험의학잡지
INTERNATIONAL JOURNAL OF LABORATORY MEDICINE
2014年
18期
2512-2514
,共3页
耐甲氧西林金黄色葡萄球菌%耐药性%SCCmec基因型%聚合酶链反应
耐甲氧西林金黃色葡萄毬菌%耐藥性%SCCmec基因型%聚閤酶鏈反應
내갑양서림금황색포도구균%내약성%SCCmec기인형%취합매련반응
methicillin-resistant Staphylococcus aureus%antimicrobial resistance%SCCmec genotype%polymerase chain re-action
目的:了解大连地区耐甲氧西林金黄色葡萄球菌(M RSA )的耐药性及其葡萄球菌盒染色体SCCmec的基因型特点。方法采用纸片扩散法检测MRSA对抗菌药物的耐药性;聚合酶链反应(PCR)对SCCmec进行基因分型。结果分离出的38株M RSA菌株均具有多重耐药性,但对万古霉素和氯霉素较为敏感。SCCmec基因型以Ⅱ型最为常见,占86.84%;其次为Ⅲ型,占10.53%;未定型的占2.6%。结论大连地区分离出的M RSA具有多重耐药的特征,SCCmec基因型以Ⅱ型为主。
目的:瞭解大連地區耐甲氧西林金黃色葡萄毬菌(M RSA )的耐藥性及其葡萄毬菌盒染色體SCCmec的基因型特點。方法採用紙片擴散法檢測MRSA對抗菌藥物的耐藥性;聚閤酶鏈反應(PCR)對SCCmec進行基因分型。結果分離齣的38株M RSA菌株均具有多重耐藥性,但對萬古黴素和氯黴素較為敏感。SCCmec基因型以Ⅱ型最為常見,佔86.84%;其次為Ⅲ型,佔10.53%;未定型的佔2.6%。結論大連地區分離齣的M RSA具有多重耐藥的特徵,SCCmec基因型以Ⅱ型為主。
목적:료해대련지구내갑양서림금황색포도구균(M RSA )적내약성급기포도구균합염색체SCCmec적기인형특점。방법채용지편확산법검측MRSA대항균약물적내약성;취합매련반응(PCR)대SCCmec진행기인분형。결과분리출적38주M RSA균주균구유다중내약성,단대만고매소화록매소교위민감。SCCmec기인형이Ⅱ형최위상견,점86.84%;기차위Ⅲ형,점10.53%;미정형적점2.6%。결론대련지구분리출적M RSA구유다중내약적특정,SCCmec기인형이Ⅱ형위주。
Objective To investigate antibiotic resistance and Staphylococcal cassette chromosome mec (SCC-mec) genotype of methicillin-resistant Staphylococcus aureus (MRSA)in Dalian Area .Methods Antimicrobial susceptibility of MRSA was detected by Kirby-Bauer method and SCCmec genotype by multiple PCR method .Results 38 strains of MRSA were all multidrug-resistant strains ,but susceptible to vancomycin and chloramphenicol .The most common SCCmec genotype was type Ⅱ (86 .84% ) ,type Ⅲwas in the second place(10 .53% ) ,and unidentified type accounted for 2 .6% .Conclusion MRSA strains in Dalian area are charac-terized by multidrug resistance ,type Ⅱ is the most common SCCmec genotype .