中国现代医生
中國現代醫生
중국현대의생
CHINA MODERN DOCTOR
2014年
26期
11-13
,共3页
miR-26a%胃癌%生长增殖
miR-26a%胃癌%生長增殖
miR-26a%위암%생장증식
miR-26a%Gastric cancer%Growth and proliferation
目的：检测miR-26a在胃癌组织的表达改变，明确miR-26a调控胃癌细胞生长的分子机制。方法运用qRT-PCR检测71例胃癌及相应癌旁正常组织中miR-26a的表达改变；将miR-26a 模拟物转染胃癌细胞AGS，采用Western blot检测其对MTDH蛋白表达水平的影响；然后采用MTT法检测高表达miR-26a对AGS细胞生长增殖的影响。结果 qRT-PCR检测结果显示，miR-26a在71例胃癌组织中表达下调0.44倍；Western blot结果显示，过表达miR-26a或干扰MTDH可抑制MTDH蛋白的表达。 MTT检测发现，转染MTDH siRNA和miR-26a模拟物组AGS细胞从48 h起OD值分别为(0.158±0.006)、(0.201±0.006)，与对照组细胞相比(0.515±0.032)、(0.479±0.028)，差异均有统计学意义(P<0.05)。结论 miR-26a通过靶向调控MTDH的表达而抑制胃癌细胞的生长。
目的：檢測miR-26a在胃癌組織的錶達改變，明確miR-26a調控胃癌細胞生長的分子機製。方法運用qRT-PCR檢測71例胃癌及相應癌徬正常組織中miR-26a的錶達改變；將miR-26a 模擬物轉染胃癌細胞AGS，採用Western blot檢測其對MTDH蛋白錶達水平的影響；然後採用MTT法檢測高錶達miR-26a對AGS細胞生長增殖的影響。結果 qRT-PCR檢測結果顯示，miR-26a在71例胃癌組織中錶達下調0.44倍；Western blot結果顯示，過錶達miR-26a或榦擾MTDH可抑製MTDH蛋白的錶達。 MTT檢測髮現，轉染MTDH siRNA和miR-26a模擬物組AGS細胞從48 h起OD值分彆為(0.158±0.006)、(0.201±0.006)，與對照組細胞相比(0.515±0.032)、(0.479±0.028)，差異均有統計學意義(P<0.05)。結論 miR-26a通過靶嚮調控MTDH的錶達而抑製胃癌細胞的生長。
목적：검측miR-26a재위암조직적표체개변，명학miR-26a조공위암세포생장적분자궤제。방법운용qRT-PCR검측71례위암급상응암방정상조직중miR-26a적표체개변；장miR-26a 모의물전염위암세포AGS，채용Western blot검측기대MTDH단백표체수평적영향；연후채용MTT법검측고표체miR-26a대AGS세포생장증식적영향。결과 qRT-PCR검측결과현시，miR-26a재71례위암조직중표체하조0.44배；Western blot결과현시，과표체miR-26a혹간우MTDH가억제MTDH단백적표체。 MTT검측발현，전염MTDH siRNA화miR-26a모의물조AGS세포종48 h기OD치분별위(0.158±0.006)、(0.201±0.006)，여대조조세포상비(0.515±0.032)、(0.479±0.028)，차이균유통계학의의(P<0.05)。결론 miR-26a통과파향조공MTDH적표체이억제위암세포적생장。
Objective To explicit the expression and role of miR-26a in gastric cancer tissues, thus to reveal molecular mechanism that miR-26a functions in gastric cancer cells. Methods The qRT-PCR was conducted for detect the ex-pression of miR-26a in 71 cases of gastric cancers. AGS cells were transfected with miR-26a mimics, then Western blot was performed to detect the expressions of MTDH protein. The proliferation ability of AGS cells evaluated by MTT assy. Results qRF-PCR showed that miR-26a was down-regulated 0.44 times in 71 cases of gastric cancer tissues. Western blot showed that the expressions of MTDH protein was inhibited by restored miR-26a or MTDH siRNA in AGS cells. Overexpression of miR-26a or MTDH siRNA inhibited the proliferation of AGS cells. Their OD values of transfected miR-26a mimics and MTDH siRNA were (0.158±0.006),(0.201±0.006) respectively, and their OD values of transfected control were (0.515±0.032),(0.479±0.028) respectively,there were statistically significance(P<0.05). Con-clusion miR-26a suppresses cell proliferation by targeting MTDH in gastric cancer.
