CAJ | 학술논문

目的：建立2型糖尿病单纯性脂肪肝大鼠模型，观察桑叶黄酮对大鼠肝组织PPARγ的影响，探讨桑叶黄酮改善胰岛素敏感性及脂肪肝发病的可能分子机制。方法用小剂量链脲佐菌素(STZ)注射加高脂饲料喂养，建立2型糖尿病单纯性脂肪肝大鼠模型；分别给予桑叶黄酮和罗格列酮喂食，测空腹血糖、胰岛素、PPARγ，观察大鼠肝组织PPARγ的变化。结果①模型对照组(MOD组)大鼠胰岛素敏感指数相对正常对照组(NOR组)显著降低，但桑叶黄酮组(MLF组)、罗格列酮组(RGZ组)比MOD组胰岛素敏感指数显著升高，而MLF组和RGZ组差异无显著性。②与NOR组相比，MOD组大鼠肝组织PPARγ表达显著减少；MLF组、RGZ组与MOD组相比，肝组织中PPARγ表达显著增加；MLF组与RGZ组相比，肝组织中的PPARγ表达差异无显著性。结论桑叶黄酮能提高2型糖尿病单纯性脂肪肝大鼠胰岛素的敏感性，改善胰岛素抵抗，其胰岛素增敏作用可能与提高肝组织中的PPARγ表达有关。
목적：건립2형당뇨병단순성지방간대서모형，관찰상협황동대대서간조직PPARγ적영향，탐토상협황동개선이도소민감성급지방간발병적가능분자궤제。방법용소제량련뇨좌균소(STZ)주사가고지사료위양，건립2형당뇨병단순성지방간대서모형；분별급여상협황동화라격렬동위식，측공복혈당、이도소、PPARγ，관찰대서간조직PPARγ적변화。결과①모형대조조(MOD조)대서이도소민감지수상대정상대조조(NOR조)현저강저，단상협황동조(MLF조)、라격렬동조(RGZ조)비MOD조이도소민감지수현저승고，이MLF조화RGZ조차이무현저성。②여NOR조상비，MOD조대서간조직PPARγ표체현저감소；MLF조、RGZ조여MOD조상비，간조직중PPARγ표체현저증가；MLF조여RGZ조상비，간조직중적PPARγ표체차이무현저성。결론상협황동능제고2형당뇨병단순성지방간대서이도소적민감성，개선이도소저항，기이도소증민작용가능여제고간조직중적PPARγ표체유관。
Objective To establish type 2 diabetic rats with simple fatty liver model,observed the efficacy effect of mulberry flavone on rat liver PPARγ,and to explore the possible molecular mechanism of fatty liver and how mulberry flavone improves insulin resistance. Methods Rats were fed with high-fatty diet,then small doses of streptozotion (STZ) were injected intraperitoneally to type 2 diabetic rats with simple fatty liver model. Then part of the rats were fed with mulberry flavone and rosiglitazone. The fasting blood glucoses, fasting insulins and PPARγ were measured.Then ob-served the variety of PPARγ in rat liver. Results ①Compared with normal group (NOR),insulin sensitivity indexes were significantly lower in model group (MOD),but it was increased in mulberry flavone (MLF) and rosiglitazone group (RGZ),and there was no significant difference between MLF and RGZ.②Compared with NOR group, the PPARγof rat liver were significantly reduced in MOD,and increased in MLF and RGZ, but also it had no significant difference be-tween MLF and RGZ. Conclusion Mulberry flavone can improve insulin sensitivity in type 2 diabetic rats with simple fatty liver, also can improve insulin resistance, and this effect is possible related about the increased PPARγof rat liver.