浙江林业科技
浙江林業科技
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JOURNAL OF ZHEJIANG FORESTRY SCIENCE AND TECHNOLOGY
2013年
5期
81-84
,共4页
郭佳%朱鹏飞%房丹%高中海%徐梁
郭佳%硃鵬飛%房丹%高中海%徐樑
곽가%주붕비%방단%고중해%서량
海滨木巴戟%组培%分化%生根
海濱木巴戟%組培%分化%生根
해빈목파극%조배%분화%생근
Morinda citrifolia%tissue culture%differentiation%rooting
以海滨木巴戟(Morinda citrifolia)的嫩芽为外植体进行组培研究,以1/2MS+蔗糖30 g/L+琼脂6 g/L为基本培养基,以TDZ、BA、IBA三种激素进行不定芽的诱导分化实验,以IBA、NAA进行生根实验。结果表明,各因素对外植体分化的影响皆不显著(p<0.05),IBA抑制外植体分化;在只添加BA的培养基中,随着BA浓度的升高,外植体玻璃化的现象有增加的趋势;生根实验结果表明,NAA和IBA诱导生根效果相差不大(p<0.05),但是IBA在外植体基部诱导出更多的愈伤组织。
以海濱木巴戟(Morinda citrifolia)的嫩芽為外植體進行組培研究,以1/2MS+蔗糖30 g/L+瓊脂6 g/L為基本培養基,以TDZ、BA、IBA三種激素進行不定芽的誘導分化實驗,以IBA、NAA進行生根實驗。結果錶明,各因素對外植體分化的影響皆不顯著(p<0.05),IBA抑製外植體分化;在隻添加BA的培養基中,隨著BA濃度的升高,外植體玻璃化的現象有增加的趨勢;生根實驗結果錶明,NAA和IBA誘導生根效果相差不大(p<0.05),但是IBA在外植體基部誘導齣更多的愈傷組織。
이해빈목파극(Morinda citrifolia)적눈아위외식체진행조배연구,이1/2MS+자당30 g/L+경지6 g/L위기본배양기,이TDZ、BA、IBA삼충격소진행불정아적유도분화실험,이IBA、NAA진행생근실험。결과표명,각인소대외식체분화적영향개불현저(p<0.05),IBA억제외식체분화;재지첨가BA적배양기중,수착BA농도적승고,외식체파리화적현상유증가적추세;생근실험결과표명,NAA화IBA유도생근효과상차불대(p<0.05),단시IBA재외식체기부유도출경다적유상조직。
The axillary buds of Morinda citrifolia were used as the explants to establish the micro-propagation system. The orthogonal experiment of bud differentiation was carried out by TDZ, BA and IBA. The results showed that three hormones had no significant(p < 0.05) effect on bud differentiation, but IBA inhibited the differentiation. The number of vitrification shoots increased with the concentration of BA when the medium was only added BA. Experiment on rooting induction demonstrated that the influences of NAA and IBA had no obvious difference (p<0.05). However, more callus were induced from the base of explant in the medium with IBA.