CAJ | 학술논문

为提高微杆菌Z4发酵的几丁质酶得率，利用摇瓶研究了发酵温度、初始p H、装液量等对于发酵产酶的影响．在这些实验的基础上，运用正交试验对Z4产几丁质酶的摇瓶发酵条件进行了进一步优化．结果的极差分析和方差分析表明温度对产酶的影响最大，具有显著性．最佳摇瓶发酵工艺条件为：培养温度28℃，培养基初始p H 7，接种量6％，三角瓶装液量60 m L培养基/250 m L三角瓶．在该条件下发酵液酶产量达到4．13 U/m L ，比优化前提高了79．6％．
위제고미간균Z4발효적궤정질매득솔，이용요병연구료발효온도、초시p H、장액량등대우발효산매적영향．재저사실험적기출상，운용정교시험대Z4산궤정질매적요병발효조건진행료진일보우화．결과적겁차분석화방차분석표명온도대산매적영향최대，구유현저성．최가요병발효공예조건위：배양온도28℃，배양기초시p H 7，접충량6％，삼각병장액량60 m L배양기/250 m L삼각병．재해조건하발효액매산량체도4．13 U/m L ，비우화전제고료79．6％．
The effect of the fermentation temperature ,inoculum ratio and liquid volume of the flask on the chitinase fermentation of Microbacterium sp Z4 was studied .The fermentation condition for optimized production of chitinase was further studied by using L9 (34 ) orthogo-nal experimental method .The maximal chitinase activity up to 4 .13 U/mL was attained un-der the condition :temperature of 28 degrees Celsius ,initial pH 7 ,inoculums 6% ,and 60 mL medium in 250 mL flask .The chitinase production increased 79 .6% of the initial production .