中南民族大学学报（自然科学版）
中南民族大學學報（自然科學版）
중남민족대학학보（자연과학판）
JOURNAL OF SOUTH-CENTRAL UNIVERSITY FOR NATIONALITIES(NATURAL SCIENCE EDITION)
2014年
1期
15-18
,共4页
乳酸脱氢酶%固定化%壳聚糖%酶催化性质
乳痠脫氫酶%固定化%殼聚糖%酶催化性質
유산탈경매%고정화%각취당%매최화성질
lactate dehydrogenase%immobilization%chitosan%enzymatic characteristics
研究了固定化乳酸脱氢酶（ LDH）的制备和催化性质，讨论了戊二醛体积分数、酶的偶联时间、pH值对酶固定化的影响．对游离酶和固定化酶催化性质的研究结果表明：酶促反应最适pH分别为9．2和10．2，最适温度分别为51℃和50℃，米氏常数分别为16．2 mmol/L和0．7 mmol/L．与游离酶相比，固定化酶的活力稳定性更佳，有更好的贮存稳定性和复用性．
研究瞭固定化乳痠脫氫酶（ LDH）的製備和催化性質，討論瞭戊二醛體積分數、酶的偶聯時間、pH值對酶固定化的影響．對遊離酶和固定化酶催化性質的研究結果錶明：酶促反應最適pH分彆為9．2和10．2，最適溫度分彆為51℃和50℃，米氏常數分彆為16．2 mmol/L和0．7 mmol/L．與遊離酶相比，固定化酶的活力穩定性更佳，有更好的貯存穩定性和複用性．
연구료고정화유산탈경매（ LDH）적제비화최화성질，토론료무이철체적분수、매적우련시간、pH치대매고정화적영향．대유리매화고정화매최화성질적연구결과표명：매촉반응최괄pH분별위9．2화10．2，최괄온도분별위51℃화50℃，미씨상수분별위16．2 mmol/L화0．7 mmol/L．여유리매상비，고정화매적활력은정성경가，유경호적저존은정성화복용성．
In this paper,the preparation and enzymatic characteristics of immobilized lactate dehydrogenase ( LDH) was studied and the impact of glutaraldehyde concentration , enzyme binding time and pH value on immobilization were discussed.The results of the study on the enzymatic characteristics of free and immobilized enzymes showed that the optimum pH value for the enzymatic reaction were 9.2 and 10.2,the optimum temperatures were 51℃ and 50℃,the Km value were 16.2 mmol/L and 0.7 mmol/L respectively.It was shown that the immobilized enzyme has better activity stability, storage stability and reusability .
