CAJ | 학술논문

建立一套稳定、灵敏度高、重现性好和耗时短的鉴定小鼠灌胃染料木素单体后体内代谢产物的分析方法，检测染料木素在小鼠血浆中代谢产物的形式与含量。12只健康雌性小鼠按200 mg·kg-1灌胃染料木素单体，血浆预处理处理后，使用超高效液相色谱（UPLC）/四极杆-飞行时间串联质谱仪（Q-TOF）检测，以体积分数2．5 mM 乙酸铵缓冲液（A）-乙腈（B）为流动相梯度洗脱，电喷雾离子源负离子条件下进行 MS1和 MS2全扫描，鉴定小鼠给药染料木素单体后血浆中的代谢产物。染料木素在小鼠血液中的代谢产物的主要存在形式是染料木素-葡糖醛酸，约占54．82％；其次是染料木素-硫酸酯，约占26．67％；也有一小部分（16．61％）以染料木素苷元的形式存在。UPLC/Q-TOF 可快速、有效地鉴定出小鼠血浆中染料木素的代谢产物，为今后进行染料木素与人类健康效应相关的研究提供参考。
건립일투은정、령민도고、중현성호화모시단적감정소서관위염료목소단체후체내대사산물적분석방법，검측염료목소재소서혈장중대사산물적형식여함량。12지건강자성소서안200 mg·kg-1관위염료목소단체，혈장예처리처리후，사용초고효액상색보（UPLC）/사겁간-비행시간천련질보의（Q-TOF）검측，이체적분수2．5 mM 을산안완충액（A）-을정（B）위류동상제도세탈，전분무리자원부리자조건하진행 MS1화 MS2전소묘，감정소서급약염료목소단체후혈장중적대사산물。염료목소재소서혈액중적대사산물적주요존재형식시염료목소-포당철산，약점54．82％；기차시염료목소-류산지，약점26．67％；야유일소부분（16．61％）이염료목소감원적형식존재。UPLC/Q-TOF 가쾌속、유효지감정출소서혈장중염료목소적대사산물，위금후진행염료목소여인류건강효응상관적연구제공삼고。
The purpose of this research was to develop a stable,sensitive and reproducible method to qualita-tively and quantitatively analyze genistein in blood samples of mice.12 healthy female mice were adminis-tered by gavage with genistein at the dose of 200 mg·kg-1 body weight.The blood samples were analyzed by tandem quadrupole-time of flight mass spectrometry in a negative ion mode based on gradient elution with a mobile phase composed of 100% aqueous buffer (2.5 mM ammonium acetate,A)and 100% acetoni-trile (B)to identify the metabolites of genistein after treatment.The data suggested that the main form of genistein in the blood samples of the genistein group were genistein-glucuronides (accounting for 54. 82%),genistein-sulfates (26.67%),and there also existed a small portion (16.61%)of genistein agly-cone.Meanwhile,these metabolites were not detected in the blood samples of control group.Our results in-dicated that UPLC/Q-TOF could quickly and effectively identify genistein metabolites in mice plasma and thus provide a novel reference for the investigation the human health effects of dietary genistein.