CAJ | 학술논문

为了解2012年上海地区副溶血性弧菌人源株和食源株的优势血清型及其毒力基因携带状况，本研究收集了2012年从上海市15个区（县）腹泻患者和食品监测中分离的副溶血性弧菌株，进行血清分型，并采用聚合酶链反应（PCR）检测 tdh和trh基因。结果显示，854株副溶血性弧菌中，88．1％为血清可分型，89．8％为产毒株。O3∶K6、O4∶K8、O1∶K25、O4∶K68、O4∶K9、O1∶K36、O3∶K29为上海地区可分型人源株的优势血清型（93．8％），其中O3∶K6最多，达56．2％。副溶血性弧菌全部分离株的月份分布显示出聚集趋势，7～8月为高峰期。O4∶K9和O1∶K36血清型菌株的月份分布与其他优势血清型菌株不同，未表现出明显聚集趋势。食源株无明显优势血清型，且与人源株分布不同。人源株产毒株构成（95．6％）高于食源株（5．5％）。人源株优势血清型产毒株构成（99．9％）高于非优势血清型（71．1％）。血清可分型人源株的 tdh携带率（97．5％）高于不可分型人源株（67．6％），血清可分型人源株的 trh携带率（0．8％）低于不可分型人源株（42．6％）。结果提示，副溶血性弧菌血清型分布与历史数据相比变化较大，血清型与毒力基因携带呈一定程度关联，且人源株与食源株在血清型和毒力基因携带上具有分离现象。因此，在副溶血性弧菌的监测与检测中应充分考虑血清分型和毒力基因的重要性。
위료해2012년상해지구부용혈성호균인원주화식원주적우세혈청형급기독력기인휴대상황，본연구수집료2012년종상해시15개구（현）복사환자화식품감측중분리적부용혈성호균주，진행혈청분형，병채용취합매련반응（PCR）검측 tdh화trh기인。결과현시，854주부용혈성호균중，88．1％위혈청가분형，89．8％위산독주。O3∶K6、O4∶K8、O1∶K25、O4∶K68、O4∶K9、O1∶K36、O3∶K29위상해지구가분형인원주적우세혈청형（93．8％），기중O3∶K6최다，체56．2％。부용혈성호균전부분리주적월빈분포현시출취집추세，7～8월위고봉기。O4∶K9화O1∶K36혈청형균주적월빈분포여기타우세혈청형균주불동，미표현출명현취집추세。식원주무명현우세혈청형，차여인원주분포불동。인원주산독주구성（95．6％）고우식원주（5．5％）。인원주우세혈청형산독주구성（99．9％）고우비우세혈청형（71．1％）。혈청가분형인원주적 tdh휴대솔（97．5％）고우불가분형인원주（67．6％），혈청가분형인원주적 trh휴대솔（0．8％）저우불가분형인원주（42．6％）。결과제시，부용혈성호균혈청형분포여역사수거상비변화교대，혈청형여독력기인휴대정일정정도관련，차인원주여식원주재혈청형화독력기인휴대상구유분리현상。인차，재부용혈성호균적감측여검측중응충분고필혈청분형화독력기인적중요성。
The main purpose of the research is to define serotype distribution and virulence factors of Vibrio parahaemolyticus (V . parahaemolyticus) isolates in Shanghai ,2012 .A total of 854 isolated strains collected through the diarrhea surveillance system from 15 district Centers for Disease Control and Prevention (739 isolates) ,food surveillance (49 strains) and suspected food poisoning cases (66 strains) were subjected to the assays for O∶ K serotypes and a polymerase chain reaction (PCR )-based detection for tdh and (or ) trh genes respectively .88 .1% of the tested isolates could be serotyped with both O and K antisera ,and 89 .8%of the strains were positive for tdh and (or ) trh genes . Among the serotypeable isolates from diarrhea cases ,the positive rates for O3∶K6 ,O4∶K8 ,O1∶K25 ,O4∶K68 ,O4∶K9 ,O1∶K36 and O3∶K29 were 56 .2% ,13 .5% ,6 .8% ,7 .0% ,4 .5% ,2 .9% and 2 .9% respectively .O3 ∶ K6 was the most common serotype in Shanghai .The serotype distribution in food isolates was different from that in diarrhea isolates . The proportion of virulent strains in the diarrhea isolates (95 .6% ) was higher than that in the food isolates (5 .5% ) .Among diarrhea isolates , the proportion of virulent strains in the most common serotypes was higher than that in the other serotypes (71 .1% ) .The tdh detection rate in the serotypeable (97 .5% ) was higher than that in the unserotypeable (67 .6% ) ,but the trh detection rate in the serotypeable (0 .8% ) was lower than that in the unserotypeable (42 .6% ) .The results hint that the serotype constitute is different from that of historical data ,and tdh ( trh) carrying or not is likely to be related to serotypes .The isolates from diarrhea cases and food show separation phenomena in serotypes and virulent factors ,indicating the importance of serotyping and virulence factor detection in surveillance and detection of V . parahaemolyticus .