CAJ | 학술논문

本研究原核表达并纯化了布鲁菌脂蛋白Omp19，并通过蛋白免疫印迹法对其免疫原性进行验证。采用Omp19作为包被抗原，建立检测羊种布鲁菌的间接酶联免疫吸附试验（iELISA），检测90份羊血清样本，并与标准血清凝集试验（SAT）比较，用SPSS软件进行数据分析。结果显示，iELISA与SAT的阳性符合率为95．12％，阴性符合率为67．35％，Kappa值为0．6077。对2种方法进行McNemar卡方检验，结果有显著性差异（P＜0．05）。本研究建立的iELISA与SAT的总符合率达80％，可作为羊种布鲁菌病血清学诊断的备用方法。
본연구원핵표체병순화료포로균지단백Omp19，병통과단백면역인적법대기면역원성진행험증。채용Omp19작위포피항원，건립검측양충포로균적간접매련면역흡부시험（iELISA），검측90빈양혈청양본，병여표준혈청응집시험（SAT）비교，용SPSS연건진행수거분석。결과현시，iELISA여SAT적양성부합솔위95．12％，음성부합솔위67．35％，Kappa치위0．6077。대2충방법진행McNemar잡방검험，결과유현저성차이（P＜0．05）。본연구건립적iELISA여SAT적총부합솔체80％，가작위양충포로균병혈청학진단적비용방법。
Brucella protein Omp19 was expressed and purified .Its immunogenicity was further validated by Western blotting .An indirect enzyme-linked immunosorbent assay (iELISA) for the detection of Brucella melitensis (B . melitensis) was established using the purified recombinant protein Omp 19 as coating antigen . Serum samples (n=90) from sheep were simultaneously tested by iELISA and standard serum agglutination test (SAT) ,and the data was analyzed by SPSS software .Compared with SAT ,the positive coincidence rate of the Omp 19 based iELISA was 95 .12% and the negative coincidence rate was 67 .35% .Kappa value was 0 .607 7 .McNemar chi-square test showed that there was significant difference between the two methods ( P<0 .05) . The total diagnosis coincidence rate was 80% . The established iELISA could be used as an alternative method for serological diagnosis of brucellosis .