CAJ | 학술논문

目的：探讨同种异体气管移植的临床效果及吻合口病理学变化。方法异体羊新鲜心包经过0.625％戊二醛处理后，包绕6 cm Sigma不锈钢丝网支架，制成人工气管。切除实验用羊颈段气管5 cm，人工气管与实验羊断端气管头尾端吻合。手术后60、120、180 d行支气管镜检查、X线片检查，定期处死实验羊行人工气管病理学检查，观察人工气管、吻合口愈合、血运及肉芽组织情况。结果人工气管可以诱导受体羊气管周围组织生成气管样组织，填充缺损区。60 d，移植区上皮组织不明显；120 d，移植区覆盖有部分纤毛柱状上皮，气管各层次比较完整；180 d，黏膜层有纤毛柱状上皮覆盖，黏膜下层、外膜等小血管丰富，移植区气管各层次完整。结论人工气管可以诱导受体羊气管周围组织生成完整的气管样组织，气管各层次比较完整，实验动物有望长期存活。
목적：탐토동충이체기관이식적림상효과급문합구병이학변화。방법이체양신선심포경과0.625％무이철처리후，포요6 cm Sigma불수강사망지가，제성인공기관。절제실험용양경단기관5 cm，인공기관여실험양단단기관두미단문합。수술후60、120、180 d행지기관경검사、X선편검사，정기처사실험양행인공기관병이학검사，관찰인공기관、문합구유합、혈운급육아조직정황。결과인공기관가이유도수체양기관주위조직생성기관양조직，전충결손구。60 d，이식구상피조직불명현；120 d，이식구복개유부분섬모주상상피，기관각층차비교완정；180 d，점막층유섬모주상상피복개，점막하층、외막등소혈관봉부，이식구기관각층차완정。결론인공기관가이유도수체양기관주위조직생성완정적기관양조직，기관각층차비교완정，실험동물유망장기존활。
Objective To research a kind of artificial trachea which could biologically fuse with autolo-gous trachea.Methods Firstly allergenic fresh sheep pericardium treated by 0.625% glutaraldehyde were used to surround a 6cm long Sigma stainless steel wire net stents to prepare the artificial trachea.Then re-sected a 5 cm long cervical trachea of the sheep and sutured the upper and lower end with the artificial tra-chea and observed the healing of artificial trachea by X-ray and bronchoscopic examination on 60,120 and 180 days after the operation.Pathological examination was performed at regular intervals to observe the healing of anastomotic stoma,blood circulation and conditions in artifical trachea to supply theoretical ba-sis for the clinic.Results The artificial trachea and autologous trachea healed together in all of 12 animals and trachea like tissues formed to fill the defect area but the change needed sufficient time.On the day 60 after the operation there was no obvious ciliated columnar epithelium in the transplanting area. However, on the day 120 after operation,there was a small quantity of ciliated columnar epithelium but the tissues were not complete.On the day 180 after operation,the tissues of trachea in transplanting area were rela-tively complete-the mucosa was covered by ciliated columnar epithelium, small blood vessels were abun-dant in the mucosa and submucosa and gland could be seen.Conclusion Artificial trachea could induce the receptor trachea tissues to form a complete trachea,each lay of the trachea is relatively complete and the experiment animals can be long-term survived.