CAJ | 학술논문

目的：探讨c-Myc、k-Ras、p16蛋白在胆囊癌细胞中的表达及临床意义。方法：收集20例胆囊癌、50例慢性胆囊炎和20例正常胆囊组织标本，用免疫组化法检测c-Myc、k-Ras、p16蛋白的表达，分析三种蛋白表达与胆囊癌的临床病理特征关系。结果：c-Myc、k-Ras蛋白定位于细胞质和细胞核中，在胆囊癌中的表达程度明显高于慢性胆囊炎和正常胆囊，差异有统计学意义（P<0.01）。p16定位于胞质，在胆囊癌中的表达程度明显低于正常胆囊和慢性胆囊炎，差异有统计学意义（P<0.01）。c-Myc表达程度与肿瘤大小、分化和淋巴结转移密切相关（P<0.05）；k-Ras表达程度与肿瘤大小、侵犯深度和淋巴结转移密切相关（P<0.05）。结论：c-Myc、k-Ras、p16蛋白在胆囊癌细胞中呈异常表达，其表达与临床病理特征相关，可能在胆囊癌发生发展中起有重要作用。
목적：탐토c-Myc、k-Ras、p16단백재담낭암세포중적표체급림상의의。방법：수집20례담낭암、50례만성담낭염화20례정상담낭조직표본，용면역조화법검측c-Myc、k-Ras、p16단백적표체，분석삼충단백표체여담낭암적림상병리특정관계。결과：c-Myc、k-Ras단백정위우세포질화세포핵중，재담낭암중적표체정도명현고우만성담낭염화정상담낭，차이유통계학의의（P<0.01）。p16정위우포질，재담낭암중적표체정도명현저우정상담낭화만성담낭염，차이유통계학의의（P<0.01）。c-Myc표체정도여종류대소、분화화림파결전이밀절상관（P<0.05）；k-Ras표체정도여종류대소、침범심도화림파결전이밀절상관（P<0.05）。결론：c-Myc、k-Ras、p16단백재담낭암세포중정이상표체，기표체여림상병리특정상관，가능재담낭암발생발전중기유중요작용。
Objective:To investigate the expressions of c-Myc, k-Ras and p16 protein in the gallbladder carcinoma cells and their clinical implication. Methods:The expressions of c-Myc, k-Ras and p16 protein were detected in 20 cases of gallbladder carcinoma, 50 cases of chronic cholecystitis and 20 cases of normal gallbladder tissue specimens with the immunohistochemical method. The relationships of the expressions of three kinds of proteins with the clinical pathological characteristics were assayed. Results:The c-Myc and k-Ras expressions were localized in the cytoplasm and nucleus of tumor cells, and the expressions in gallbladder carcinoma were signiifcantly higher than those in chronic cholecystitis and the normal gallbladder, whose difference had the statistical signiifcance (P<0.01). The location of p16 protein was found in the cytoplasm. The expression degrees in the gallbladder carcinoma were obviously lower than those in the normal gallbladder and chronic cholecystitis, whose difference had the statistic signiifcance (P<0.01). The expression degree of c-Myc was signiifcant correlation with the size of the tumor, poor differentiation, and lymphatic metastasis (P<0.05). The expression degree of k-Ras was significant correlation with the size of the tumor, depth of invasion, and lymphatic metastasis (P<0.05). Conclusion:The expressions of c-Myc, k-Ras, and p16 protein present abnormal in the gallbladder carcinoma cells and their expressions are correlated with the clinical pathological characteristics which may probably play an important role in the occurrence and development of the gallbladder carcinoma.