CAJ | 학술논문

建立了分散固相萃取-超高效色谱-电喷雾串联质谱快速测定鸡肉中盐霉素残留的方法。样品经乙腈提取后，采用QuEChERS方法净化，氮吹转换溶剂后，采用Acquity BEH C18柱进行分离，0.3%甲酸水溶液-甲醇体系为流动相，梯度洗脱，电喷雾质谱正离子多反应监测模式测定，外标法定量。结果表明，在优化的条件下对样品进行分析检测，盐霉素的检出限为0.03μg·kg-1，添加水平为5.5～31.4μg·kg-1时，加标回收率为78.2-97.8%，RSD<2.0%（n=6），在0.630～25.2μg·L-1质量浓度范围内呈良好线性，相关系数R2>0.9989。该方法前处理步骤简单，具有较高灵敏度，可用于大批量样品的快速筛查检测。
건립료분산고상췌취-초고효색보-전분무천련질보쾌속측정계육중염매소잔류적방법。양품경을정제취후，채용QuEChERS방법정화，담취전환용제후，채용Acquity BEH C18주진행분리，0.3%갑산수용액-갑순체계위류동상，제도세탈，전분무질보정리자다반응감측모식측정，외표법정량。결과표명，재우화적조건하대양품진행분석검측，염매소적검출한위0.03μg·kg-1，첨가수평위5.5～31.4μg·kg-1시，가표회수솔위78.2-97.8%，RSD<2.0%（n=6），재0.630～25.2μg·L-1질량농도범위내정량호선성，상관계수R2>0.9989。해방법전처리보취간단，구유교고령민도，가용우대비량양품적쾌속사사검측。
A method for rapid determination of salinomycin in chicken was developed by ultra high performance liquid chromatography-electrospray ionization tandem mass spectrometry with dispersive solid-phase extraction. The compounds were extracted from chicken tissue with acetonitrile and then cleaned up by dispersive solid phase extraction, after solvent exchanged, the salinomycin was analyzed by LC-MS/MS on an Acquity BEH C18 column with methanol-0.3%FA-H2O solution as mobile phase by gradient elution under positive ion multiple reaction monitoring(MRM)mode. It showed, under optimized conditions the limit of detection for salinomycin was 0.03μg·kg-1, the recoveries between 5.5~31.4μg·kg-1spiked levels ranged from 78.2-97.8%, with RSDs less than 2.0%(n=6). The calibration curves was linear in the ranges of 0.630~25.2 μg·L-1, with a correlation coefficient more than 0.9989. Owe to its simple pretreatment, high sensitivity, this method could be used for rapid screening for detection of large quantities of samples.