CAJ | 학술논문

本文建立了尿中γ-羟基丁酸的水相催化衍生化-液相色谱-紫外检测联用分析方法。尿样中直接加入衍生化试剂α-溴苯乙酮、催化剂四丁基溴化铵，在80℃水浴中加热30 min，衍生化产物直接进行液相色谱-紫外检测。尿中γ-羟基丁酸浓度在0.5μg/mL-250μg/mL之间具有良好的线性关系，最低检出限为0.20μg/mL。该方法具有较好的灵敏度、准确性和精密度，能够满足司法鉴定工作的要求，具有一定的实用性。
본문건립료뇨중γ-간기정산적수상최화연생화-액상색보-자외검측련용분석방법。뇨양중직접가입연생화시제α-추분을동、최화제사정기추화안，재80℃수욕중가열30 min，연생화산물직접진행액상색보-자외검측。뇨중γ-간기정산농도재0.5μg/mL-250μg/mL지간구유량호적선성관계，최저검출한위0.20μg/mL。해방법구유교호적령민도、준학성화정밀도，능구만족사법감정공작적요구，구유일정적실용성。
A method of analysis of gamma-hydroxybutyric acid(GHB)in human urine was presented with pre-column derivatization by high performance liquid chromatography-ultraviolet detection. 2-bromoacetophenone was using as derivative reagent and tetrabutyl ammonium bromide as activator,the derivative of GHB in urine was directly analyzed by high performance liquid chromatography-ultraviolet detection. The experimental results show that the calibration curves showed good linearity in the range of 0.50μg/mL-250.0μg/mL and the limit of detection was 0.20μg/mL with pre-column derivatization and high performance liquid chromatography-ultraviolet detection of GHB in human urine. The results show high accuracy,good precision and sensitivity,and it can be widely applied in the identification of GHB in cases.