CAJ | 학술논문

目的：探讨糖尿病大鼠的高血糖状态持续时间对大鼠体内细胞免疫功能的影响。方法采用链脲佐菌素注射成功制备糖尿病大鼠模型共20只，随机分为四组，每组5只。 A组：成模后血糖升高4 d皮下注射胰岛素，控制血糖在8～12 mmol/L之间；B组：成模后血糖升高持续8 d皮下注射胰岛素控制血糖；C组：成模后血糖升高持续2周皮下注射胰岛素控制血糖；D组：未控制血糖。以正常血糖大鼠5只为正常对照组。对糖尿病大鼠分别于4 d、8 d、15 d及4周取外周血用流式细胞仪检测CD4＋、CD8＋、CD4＋/CD8＋及CD4＋CD25＋/CD4＋T淋巴细胞比率，以评价不同高血糖持续状态对正常机体细胞免疫功能的影响。结果（1）与正常对照组相比，A组及B组大鼠4、8、15 d及4周的各项细胞免疫指标无明显变化。（2） C组大鼠在15 d外周血CD4＋比例下降，CD8＋比例升高，CD4＋/CD8＋比值明显下降（ P＜0.05）。经控制血糖后，C组大鼠在4周时外周血CD4＋比例较15 d时明显升高（P＜0.05），但仍明显低于正常对照组（P＜0.05）。（3）D组大鼠在15 d及4周外周血CD4＋比例下降，CD8＋比例升高，CD4＋/CD8＋比值明显下降（P＜0.05）。（4）各组大鼠各阶段的CD4＋CD25＋/CD4＋T淋巴细胞比值与正常对照组大鼠差异无统计学意义（ P＞0.05）。结论高血糖持续时间超过2周以上即可对大鼠体内细胞免疫功能产生部分影响。在高血糖持续时间超过2周后糖尿病大鼠体内细胞免疫功能明显紊乱，表明长期高血糖可以对糖尿病个体免疫功能产生较大影响。
목적：탐토당뇨병대서적고혈당상태지속시간대대서체내세포면역공능적영향。방법채용련뇨좌균소주사성공제비당뇨병대서모형공20지，수궤분위사조，매조5지。 A조：성모후혈당승고4 d피하주사이도소，공제혈당재8～12 mmol/L지간；B조：성모후혈당승고지속8 d피하주사이도소공제혈당；C조：성모후혈당승고지속2주피하주사이도소공제혈당；D조：미공제혈당。이정상혈당대서5지위정상대조조。대당뇨병대서분별우4 d、8 d、15 d급4주취외주혈용류식세포의검측CD4＋、CD8＋、CD4＋/CD8＋급CD4＋CD25＋/CD4＋T림파세포비솔，이평개불동고혈당지속상태대정상궤체세포면역공능적영향。결과（1）여정상대조조상비，A조급B조대서4、8、15 d급4주적각항세포면역지표무명현변화。（2） C조대서재15 d외주혈CD4＋비례하강，CD8＋비례승고，CD4＋/CD8＋비치명현하강（ P＜0.05）。경공제혈당후，C조대서재4주시외주혈CD4＋비례교15 d시명현승고（P＜0.05），단잉명현저우정상대조조（P＜0.05）。（3）D조대서재15 d급4주외주혈CD4＋비례하강，CD8＋비례승고，CD4＋/CD8＋비치명현하강（P＜0.05）。（4）각조대서각계단적CD4＋CD25＋/CD4＋T림파세포비치여정상대조조대서차이무통계학의의（ P＞0.05）。결론고혈당지속시간초과2주이상즉가대대서체내세포면역공능산생부분영향。재고혈당지속시간초과2주후당뇨병대서체내세포면역공능명현문란，표명장기고혈당가이대당뇨병개체면역공능산생교대영향。
Objective To investigate the influence of the duration of hyperglycemia on cellular immune fun-tion in diabetic rat .Methods Animal model of diabetes mellitus was establish via continuously intraperitoneal injec -tion of streptozotocin .Twenty rats with successful modeling were randomly divided into 4 groups,each group include five rats.Group A:on the 4th day of successful modeling ,insulin was injected to control glucose between 8~12 mmol/L. Group B:After 8 d of successful modeling ,insulin was injected to control glucose .Group C: After 2 weeks of suc-cessful modeling ,insulin was injected to control glucose .Group D:After successful modeling , blood glucose did not controlled in entire experiment course .5 normal rats were used as control group at the same time .Peripheral blood cells were collected at 5 d, 8 d, 15 d and 4 weeks.The ratio of the CD4 +,CD8 +,CD4+/CD8 +,CD4 +CD25 +/CD4 +T cells were detected by FCM .Results ( 1 ) It showed no significant change of the ratio of CD 4 +, CD8 +, CD4 +/CD8+,CD4 +CD25 +/CD4 +T cells on 4 d, 8 d, 15 d and 4 weeks in group A and group B when compared with the control group.(2)In group C,there was a significant decrease of CD4 +T lymphocytes on 15 d,and the pro-portion of CD8 +T cell increased instead .The ratio of CD4 +/CD8+had a significant decrease ( P<0.05 ) .The ratio of the CD4 +T cells had significant increased between 15 d and 4 weeks after controlling glucose , but it was still obvi-ously lower than that of control group(P<0.05).(3)In group D,there was a significant decrease of CD4+T lym-phocytes on 15 d,and the proportion of CD 8 +T cell increased .The ratio of CD4 +/CD8 +also had a significant de-crease(P<0.05).(4)The ratio of CD4 +CD25 +/CD4 +T cells hadn′t a significant difference between experimental groups and control group ( P>0.05 ) .Conclusion Diabetic rats have cellular immune dysfunction mediated by hy-perglycemia with duration time more than two weeks .It means that chronic hyperglycemia have great effect on cellular immune function .