CAJ | 학술논문

目的：比较肝癌高发家系患者和无癌家系患者血清蛋白质谱图，探寻两者间的血清蛋白差异。方法用表面增强激光解吸电离-飞行时间质谱技术（ SELDI-TOF-MS）及CM10蛋白芯片对25例肝癌高发家系患者和25例无癌家系患者血清样本进行分析，将获得的血清蛋白质指纹图谱，用计算机软件进行比较分析，建立高发家系患者和无癌家系患者的比较筛选模型。结果肝癌高发家系组与无癌家系组共有15个蛋白质差异有统计学意义。筛选出4个蛋白峰建立诊断模型的灵敏度为96.0％（24/25），特异度为88.0％（22/25），准确率为92.0％（46/50）。结论肝癌高发家系患者组和无癌家系患者组的血清存在一定的差异蛋白， SELDI技术在特异性蛋白生物标志分子的筛选等方面具有较好的临床应用前景。
목적：비교간암고발가계환자화무암가계환자혈청단백질보도，탐심량자간적혈청단백차이。방법용표면증강격광해흡전리-비행시간질보기술（ SELDI-TOF-MS）급CM10단백심편대25례간암고발가계환자화25례무암가계환자혈청양본진행분석，장획득적혈청단백질지문도보，용계산궤연건진행비교분석，건립고발가계환자화무암가계환자적비교사선모형。결과간암고발가계조여무암가계조공유15개단백질차이유통계학의의。사선출4개단백봉건립진단모형적령민도위96.0％（24/25），특이도위88.0％（22/25），준학솔위92.0％（46/50）。결론간암고발가계환자조화무암가계환자조적혈청존재일정적차이단백， SELDI기술재특이성단백생물표지분자적사선등방면구유교호적림상응용전경。
Objective To compare serum protein pattern of patients with liver cancer from high-risk families for liver cancer with that of patients with liver cancer from no-risk familial pedigree for liver cancer and detect specific biomarkers in serum between two groups .Methods SELDI-TOF-MS Protein Chip was used to detect serum protein patterns of 25 patients with liver cancer from high-risk familial pedigree for liver cancer and 25 patients with liver cancer from no-risk families for liver cancer .Diagnostic model was developed and validated by using BPS .Results At the molecular weight range from 2 000 to 50 000 ,15 protein peaks were obviously different between two groups .Ana-lyzed by BPS, the diagnostic model′s accuracy was 92.0%(46/50),sensitivity was 96.0%(24/25) and specificity was 88.0%(22/25).Conclusion There are amount of the special serum differential proteins between two groups ,SELDI-TOF-MS Protein Chip array technology has better clinical value in detecting the special serum protein biomarkers .