CAJ | 학술논문

目的：利用代谢组学手段检测大脑中动脉阻塞（Middle Cerebral Artery Occlusion，MCAO）模型大鼠血清中氨基酸含量，微观上阐明氨基酸注射液的疗效以及部分作用机制。方法：采用邻苯二甲醛（O-Phthalaldehyde，OPA）柱前在线衍生反相高效液相色谱法，采用荧光检测，梯度洗脱，建立了血清中氨基酸代谢谱的相对定量分析方法，测定36例大鼠血清中的15种氨基酸含量。流动相A为10 mol/L Na2HP04-Na2B07缓冲液（pH＝7.95），B为乙腈-甲醇-水（45∶45∶15，V/V/V），线性梯度洗脱，流动相B在6 min内由5％增加至17.2％，6～35 min内由17.2％增加至52％。结果：1）该法在35 min之内分离出15种氨基酸；2）经主成分分析，3个组在空间上得到很好地分离，氨基酸代谢谱发生了一定的变化；3）利用SPSS方差分析，有6个氨基酸在分离各组有统计学意义。结论：丹红注射液通过部分调节MCAO摄动通路改善脑梗死大鼠血清部分氨基酸的代谢趋势。
목적：이용대사조학수단검측대뇌중동맥조새（Middle Cerebral Artery Occlusion，MCAO）모형대서혈청중안기산함량，미관상천명안기산주사액적료효이급부분작용궤제。방법：채용린분이갑철（O-Phthalaldehyde，OPA）주전재선연생반상고효액상색보법，채용형광검측，제도세탈，건립료혈청중안기산대사보적상대정량분석방법，측정36례대서혈청중적15충안기산함량。류동상A위10 mol/L Na2HP04-Na2B07완충액（pH＝7.95），B위을정-갑순-수（45∶45∶15，V/V/V），선성제도세탈，류동상B재6 min내유5％증가지17.2％，6～35 min내유17.2％증가지52％。결과：1）해법재35 min지내분리출15충안기산；2）경주성분분석，3개조재공간상득도흔호지분리，안기산대사보발생료일정적변화；3）이용SPSS방차분석，유6개안기산재분리각조유통계학의의。결론：단홍주사액통과부분조절MCAO섭동통로개선뇌경사대서혈청부분안기산적대사추세。
Objective:To determine the content of amino acid to clarify the curative effect and the functioning mechanism of DanHong injection on microscopic .Methods:OPA reversed phase high performance liquid chromatography ( HPLC ) , precolumn derivatization method fluorescence detection, gradient elution were used.Mobile phase A was 15mol/L Na2HP04-Na2B07 buffer (pH =7.95), B was acetonitrile -methanol -water (45:45:15, V/V/V).As for linear gradient elution, mobile phase B in 6 min increased from 5%to 17. 2%, increased from 17.2%to 52%in 6-35 min.Results:1) Fifteen kinds of amino acids were isolated within 35 min;2) using princi-pal component analysis , three groups were well separated;3) using the SPSS analysis of variance , there were six amino acids in the sep-aration between groups was statistically significant .Conclusion:Danhong injection can improve metabolic trend of amino acid in cerebral infarction rats serum through adjusting perturbed pathways.