CAJ | 학술논문

目的：通过对比高脂、正常大鼠胆汁流量、胆汁酸组分含量变化，初步揭示“胆道不利是肥胖发病中心环节”假说的内在依据。方法：高脂喂养造成大鼠营养性肥胖模型；实施胆管插管手术，引流胆汁，观测、对比胆汁流量差异；利用UPLC-MS技术观测、对比各胆汁酸亚组分含量差异。结果：高脂组大鼠总胆汁流量及各测试点胆汁流量均低于正常组；高脂大鼠机体内具有利胆、促进脂肪代谢作用的胆汁酸含量显著降低。结论：过量摄入高脂饮食，会加重胆代谢脂质的负担，造成胆道不利、胆汁不畅、胆汁酸组分改变，脂质代谢长期失衡，久则积聚，发为肥胖。
목적：통과대비고지、정상대서담즙류량、담즙산조분함량변화，초보게시“담도불리시비반발병중심배절”가설적내재의거。방법：고지위양조성대서영양성비반모형；실시담관삽관수술，인류담즙，관측、대비담즙류량차이；이용UPLC-MS기술관측、대비각담즙산아조분함량차이。결과：고지조대서총담즙류량급각측시점담즙류량균저우정상조；고지대서궤체내구유리담、촉진지방대사작용적담즙산함량현저강저。결론：과량섭입고지음식，회가중담대사지질적부담，조성담도불리、담즙불창、담즙산조분개변，지질대사장기실형，구칙적취，발위비반。
Objective:By comparing the difference of bile flow and bile acid components between high -fat and normal rats , we tried to reveal the basis of hypothesis which biliary tract adverseness is the central link of obesity .Methods:Building nutritional obesity rat mod-el by high fat diet .Bile duct intubation operation was implemented to collect bile and then compare the bile flow difference .UPLC-MS was used to compare the content of different kinds of bile acid components .Results: Both total and individual test point bile flow of obese rats were lower than those of normal rats .The content of bile acids that had cholagogic effect and promoting fat metabolism in high -fat diet rats reduced significantly .Conclusion:Intaking excessive high-fat diet can increase the burden of bile , resulting in biliary tract adverseness , an unsmooth flow and changes of bile acid components .Long-term imbalance of lipid metabolism will finally leads to obesi-ty.