浙江医学
浙江醫學
절강의학
ZHEJIANG MEDICAL JOURNAL
2014年
5期
401-404
,共4页
前列腺增生%A型肉毒毒素%缺氧诱导因子- 1α%血管内皮细胞生长因子
前列腺增生%A型肉毒毒素%缺氧誘導因子- 1α%血管內皮細胞生長因子
전렬선증생%A형육독독소%결양유도인자- 1α%혈관내피세포생장인자
Prostatic hyperplasia%Type A botulinum toxin%Hypoxia inducible factor- 1α%Vascular endothelial growth factor
目的：探讨A型肉毒毒素（BTX- A）对大鼠前列腺增生组织中缺氧诱导因子-1α（HIF-1α）和血管内皮细胞生长因子（VEGF）表达的影响。方法取60只大鼠，实验组45只，空白对照组15只。实验组大鼠均制作前列腺增生模型，1周后两组均随机处死5只大鼠观察建模是否成功。实验组剩余大鼠随机分为高剂量组（注射10U/0.1ml BTX- A）、中剂量组（注射6U/0.1ml BTX- A）、低剂量组（注射2U/0.1ml BTX- A）、阴性对照组（注射0.1ml0.9%氯化钠溶液），术后各组继续给予长效雄激素（十一酸睾酮针4mg· kg-1·d-1）维持，空白对照组仍给予等量橄榄油皮下注射。4周后处死各组大鼠，取出前列腺称重，免疫组化法检测比较各组大鼠前列腺组织中HIF-1α和VEGF的表达情况。结果大鼠前列腺增生模型建立成功。实验组大鼠HIF-1α在细胞质与细胞核均有表达，在新生血管周围表达明显增强，而VEGF的表达主要在细胞质中。与空白对照组比较，其他各组大鼠前列腺湿重均发生明显变化，差异均有统计学意义（均P＜0.05）；与阴性对照组比较，低、中、高剂量组大鼠前列腺湿重均发生明显增加，差异均有统计学意义（均P＜0.05）。空白组及阴性对照组均未见HIF-1α的表达；与空白对照组比较，高、中、低剂量组VEGF及HIF-1α的阳性表达率及灰度值、阴性对照组VEGF灰度值均发生明显变化（均P＜0.05）；与阴性对照组比较，中、低剂量组VEGF及HIF-1α的阳性表达率和高、中、低剂量组VEGF及HIF-1α的灰度值均发生明显变化（均P＜0.05）；与低剂量组比较，高、中剂量组VEGF及HIF-1α的阳性表达率及灰度值均发生明显变化（均P＜0.05）；与中剂量组比较，高剂量组VEGF的阳性表达率及灰度值和HIF-1α的阳性表达率均发生明显变化（均P＜0.05）。实验组大鼠HIF-1α与VEGF的表达呈明显正相关关系（r=0.575，P＜0.01）。结论前列腺内注射BTX- A可以通过抑制HIF-1α和VEGF的表达，从而抑制前列腺组织的血管形成，促使前列腺缩小。
目的：探討A型肉毒毒素（BTX- A）對大鼠前列腺增生組織中缺氧誘導因子-1α（HIF-1α）和血管內皮細胞生長因子（VEGF）錶達的影響。方法取60隻大鼠，實驗組45隻，空白對照組15隻。實驗組大鼠均製作前列腺增生模型，1週後兩組均隨機處死5隻大鼠觀察建模是否成功。實驗組剩餘大鼠隨機分為高劑量組（註射10U/0.1ml BTX- A）、中劑量組（註射6U/0.1ml BTX- A）、低劑量組（註射2U/0.1ml BTX- A）、陰性對照組（註射0.1ml0.9%氯化鈉溶液），術後各組繼續給予長效雄激素（十一痠睪酮針4mg· kg-1·d-1）維持，空白對照組仍給予等量橄欖油皮下註射。4週後處死各組大鼠，取齣前列腺稱重，免疫組化法檢測比較各組大鼠前列腺組織中HIF-1α和VEGF的錶達情況。結果大鼠前列腺增生模型建立成功。實驗組大鼠HIF-1α在細胞質與細胞覈均有錶達，在新生血管週圍錶達明顯增彊，而VEGF的錶達主要在細胞質中。與空白對照組比較，其他各組大鼠前列腺濕重均髮生明顯變化，差異均有統計學意義（均P＜0.05）；與陰性對照組比較，低、中、高劑量組大鼠前列腺濕重均髮生明顯增加，差異均有統計學意義（均P＜0.05）。空白組及陰性對照組均未見HIF-1α的錶達；與空白對照組比較，高、中、低劑量組VEGF及HIF-1α的暘性錶達率及灰度值、陰性對照組VEGF灰度值均髮生明顯變化（均P＜0.05）；與陰性對照組比較，中、低劑量組VEGF及HIF-1α的暘性錶達率和高、中、低劑量組VEGF及HIF-1α的灰度值均髮生明顯變化（均P＜0.05）；與低劑量組比較，高、中劑量組VEGF及HIF-1α的暘性錶達率及灰度值均髮生明顯變化（均P＜0.05）；與中劑量組比較，高劑量組VEGF的暘性錶達率及灰度值和HIF-1α的暘性錶達率均髮生明顯變化（均P＜0.05）。實驗組大鼠HIF-1α與VEGF的錶達呈明顯正相關關繫（r=0.575，P＜0.01）。結論前列腺內註射BTX- A可以通過抑製HIF-1α和VEGF的錶達，從而抑製前列腺組織的血管形成，促使前列腺縮小。
목적：탐토A형육독독소（BTX- A）대대서전렬선증생조직중결양유도인자-1α（HIF-1α）화혈관내피세포생장인자（VEGF）표체적영향。방법취60지대서，실험조45지，공백대조조15지。실험조대서균제작전렬선증생모형，1주후량조균수궤처사5지대서관찰건모시부성공。실험조잉여대서수궤분위고제량조（주사10U/0.1ml BTX- A）、중제량조（주사6U/0.1ml BTX- A）、저제량조（주사2U/0.1ml BTX- A）、음성대조조（주사0.1ml0.9%록화납용액），술후각조계속급여장효웅격소（십일산고동침4mg· kg-1·d-1）유지，공백대조조잉급여등량감람유피하주사。4주후처사각조대서，취출전렬선칭중，면역조화법검측비교각조대서전렬선조직중HIF-1α화VEGF적표체정황。결과대서전렬선증생모형건립성공。실험조대서HIF-1α재세포질여세포핵균유표체，재신생혈관주위표체명현증강，이VEGF적표체주요재세포질중。여공백대조조비교，기타각조대서전렬선습중균발생명현변화，차이균유통계학의의（균P＜0.05）；여음성대조조비교，저、중、고제량조대서전렬선습중균발생명현증가，차이균유통계학의의（균P＜0.05）。공백조급음성대조조균미견HIF-1α적표체；여공백대조조비교，고、중、저제량조VEGF급HIF-1α적양성표체솔급회도치、음성대조조VEGF회도치균발생명현변화（균P＜0.05）；여음성대조조비교，중、저제량조VEGF급HIF-1α적양성표체솔화고、중、저제량조VEGF급HIF-1α적회도치균발생명현변화（균P＜0.05）；여저제량조비교，고、중제량조VEGF급HIF-1α적양성표체솔급회도치균발생명현변화（균P＜0.05）；여중제량조비교，고제량조VEGF적양성표체솔급회도치화HIF-1α적양성표체솔균발생명현변화（균P＜0.05）。실험조대서HIF-1α여VEGF적표체정명현정상관관계（r=0.575，P＜0.01）。결론전렬선내주사BTX- A가이통과억제HIF-1α화VEGF적표체，종이억제전렬선조직적혈관형성，촉사전렬선축소。
Objective To investigate the effect of botulinum toxin type A (BTX- A) on the expression of HIF- 1a and VEGF in rat prostatic tissues of hyperplasia. Methods Sixty Wistar rats were randomly divided into experimental (n=45) and control (n=15) groups. Orchietectomy was performed in experimental group and androgen 4mg·kg-1·d-1 was administrated; the sham operation was performed in control group and liver oil was given instead. Five rats were sacrificed in each group to observe the hyperplasia in prostate glands after 1 week. Different doses of BTX- A were injected in the prostate of the castrated rats in H- dose (10U/0.1ml, n=10), M- dose (6U/0.1ml, n=10), L- dose (2U/0.1ml, n=10) and NS (NS/0.1ml, n=10) groups. After 4 weeks rats were sacrificed and the prostate glands were taken for weighting, the expression of HIF- 1α and VEGF were analyzed by immunohistochemical technique. Results The HIF- 1αdetected in cytoplasm and nucleus, especial y around the new vessels, the VEGF was detected only in cytoplasm. The weight of prostate in 4 experimental groups were significant higher than that in control group (P<0.05). The positive rate and grey value of HIF- 1αand VEGF in H- , M- and L- dose groups and VEGF in NS group were significantly higher than those in control group (P<0.05). The positive rate and grey values of HIF- 1αand VEGF in H- , M- and L- dose groups were significantly higher than those in NS group (P<0.05). There were also significant differences in positive rate and grey values of HIF- 1αand VEGF among H- , M- and L- dose groups (P<0.05). The HIF- 1αexpression was closely correlated with VEGF expression (r=0.575, P<0.01). Conclusion BTX- A can regulate the expression of HIF- 1α and VEGF of prostatic tissues, resulting in the inhibition of vascularization and attenuating hyperplasia of prostate gland.
