CAJ | 학술논문

目的：探讨氯喹对体外培养大鼠神经干细胞增殖及凋亡的影响,为氯喹作为神经系统疾病治疗药物的研究提供实验依据。方法将体外培养的大鼠神经干细胞分为对照组、氯喹(10、25、50、75mmol/l )组,用Mtt法检测大鼠神经干细胞的增殖率,用免疫组化方法检测氯喹对大鼠神经干细胞凋亡的影响。结果与对照组比较,25、50、75mmol/l氯喹处理神经干细胞细胞增殖活性随着剂量增加而逐渐下降( P<0.05),以75mmol/l氯喹抑制作用最强( P<0.01)；与对照组比较,氯喹处理（75mmol/l）组培养3天的神经干细胞的凋亡率有统计学意义( P<0.05)。结论氯喹具有抑制神经干细胞增殖及诱导神经干细胞凋亡的作用。
목적：탐토록규대체외배양대서신경간세포증식급조망적영향,위록규작위신경계통질병치료약물적연구제공실험의거。방법장체외배양적대서신경간세포분위대조조、록규(10、25、50、75mmol/l )조,용Mtt법검측대서신경간세포적증식솔,용면역조화방법검측록규대대서신경간세포조망적영향。결과여대조조비교,25、50、75mmol/l록규처리신경간세포세포증식활성수착제량증가이축점하강( P<0.05),이75mmol/l록규억제작용최강( P<0.01)；여대조조비교,록규처리（75mmol/l）조배양3천적신경간세포적조망솔유통계학의의( P<0.05)。결론록규구유억제신경간세포증식급유도신경간세포조망적작용。
Objective to investigate the effects of chloroquine on proliferation and apoptosis of rat neural stem cells in vitro, and to provide experimental basis for chloroquine in the research and treatment of diseases of the nervous system drugs.Methods the rat neural stem cells in vitro were divided into control group, chloroquine (10, 25, 50, 75mmol/l) group, using Mtt method for detection of rat neural stem cell proliferation rate , immunohistochemical method was used to detect the effect of chloroquine on apoptosis of rat neural stem.Results compared with the control group, 25, 50, 75mmol/l chloroquine treatment of neural stem cell proliferation activity with the increase of the dose decreased gradually (P<0.05), the strongest inhibitory effect on 75mmol/l of chloroquine (P<0.01);compared with the control group, chloroquine treatment (75mmol/l) apoptosis after 3 days of tissue culture of neural stem cell rate statistical signiifcance (P<0.05).Conclusion chloroquine inhibits the proliferation of neural stem cells and can induce apoptosis of neural stem cells.