CAJ | 학술논문

目的：探讨PRF和SVF对自体脂肪颗粒移植的作用，并分析这种可注射复合生物材料的移植效果．方法：健康新西兰家兔24只，分为四组：单纯脂肪组织移植组（2 mL AG＋0．2 mL NS）、脂肪颗粒复合富血小板纤维蛋白（2 mL AG＋0．2 mL PRF）组、脂肪颗粒复合基质血管成分（2 mL AG＋0．2 mL SVF）组和脂肪颗粒复合PRF和SVF[2 mL AG＋0．2 mL （SVF＋PRF）]组．术后1、3、6月取材，进行大体观察、组织病理学检测、游标卡尺和B超对移植脂肪组织的体积测量．采用SPSS16．0软件，对四组移植脂肪组织的存活率及吸收率进行方差分析并采用LSD法对不同时间点组间进行比较．结果：24周后各组植入脂肪组织的吸收率分别为 AG ＋NS [（49．4±9．5）％]，AG＋SVF [（27．2±4．4）％]，AG＋PRF [（36．4±8．5）％]和AG＋SVF＋PRF [（17．4±6．2）％]，并有显著差异（P＜0．01）．结论：实验结果表明自体脂肪组织复合PRF和SVF能够促进移植脂肪组织的再生，为临床脂肪组织移植提供一种新的策略．
목적：탐토PRF화SVF대자체지방과립이식적작용，병분석저충가주사복합생물재료적이식효과．방법：건강신서란가토24지，분위사조：단순지방조직이식조（2 mL AG＋0．2 mL NS）、지방과립복합부혈소판섬유단백（2 mL AG＋0．2 mL PRF）조、지방과립복합기질혈관성분（2 mL AG＋0．2 mL SVF）조화지방과립복합PRF화SVF[2 mL AG＋0．2 mL （SVF＋PRF）]조．술후1、3、6월취재，진행대체관찰、조직병이학검측、유표잡척화B초대이식지방조직적체적측량．채용SPSS16．0연건，대사조이식지방조직적존활솔급흡수솔진행방차분석병채용LSD법대불동시간점조간진행비교．결과：24주후각조식입지방조직적흡수솔분별위 AG ＋NS [（49．4±9．5）％]，AG＋SVF [（27．2±4．4）％]，AG＋PRF [（36．4±8．5）％]화AG＋SVF＋PRF [（17．4±6．2）％]，병유현저차이（P＜0．01）．결론：실험결과표명자체지방조직복합PRF화SVF능구촉진이식지방조직적재생，위림상지방조직이식제공일충신적책략．
AIM:This study was to explore the role of PRF and SVF on autologous adipose granule transplant,and analyze the effect of the transplantation of the injectable compound biological material.METHODS:24 healthy New Zealand rabbits were di-vided into four groups:2 ml adipose granules and 0.2 ml normal saline solution (AG+NS group),2 ml adipose granules and 0.2 ml SVF (AG+SVF group),2 ml adipose granules and 0.2 ml PRF (AG+PRF group)or 2 ml adipose granules combined with 0.1 ml SVF and 0.1 ml PRF (AG+SVF+PRF group).1,3,6 months after the operation,each group was taken gross observa-tion,histological examination,and was measured the volume of transplanted adipose tissue by Vernier caliper and B ultrasound. All data collected were expressed as means ±standard deviation (SD).Statistical analysis was performed by One-Way ANOVA and LSD method using SPSS 16.0 software.A p-value less than 0.05 was considered statistically significant.RESULTS:Twenty-four weeks post implantation,the resorption rates of implanted tis-sue in each group were (49.4 ±9.5%),(27.2 ±4.4%),(36. 4 ±8.5%)and (17.4 ±6.2%),respectively,and were signifi-cantly different (P<0.01).CONCLUSION:The results dem-onstrated that the efficacy of adipose tissue implantation can be enhanced by using autologous PRF and SVF cells as therapeutic adjuvants,offering a clinically translatable strategy for augmenting soft tissue reconstruction.