中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2014年
6期
34-35
,共2页
肝细胞癌%癌-睾丸抗原%SSX2%逆转录-聚合酶链反应
肝細胞癌%癌-睪汍抗原%SSX2%逆轉錄-聚閤酶鏈反應
간세포암%암-고환항원%SSX2%역전록-취합매련반응
Hepatocellular carcinoma (HCC)%Cancer-testis antigen%Synovial sarcoma X break-point2 (SSX2)%RT-PCR
目的:检测癌-睾丸抗原SSX2在肝细胞癌组织中的表达。方法应用RT-PCR技术,对40例肝细胞癌的癌组织及相应癌旁组织进行SSX2基因的mRNA检测。结果肝细胞癌组织中SSX2 mRNA表达阳性率为27.5%(11/40),而相应的癌旁组织无SSX2 mRNA表达阳性率为0(0/40),两组比较差异有统计学意义(P<0.001)。统计学分析显示SSX2表达与AFP阳性率、HbsAg阳性率、病理分级、肝细胞癌肿瘤大小、肝细胞癌是否伴有肝硬化的情况等相关的临床指标均无关(P>0.05)。结论 SSX2在HCC中呈低频率的表达。
目的:檢測癌-睪汍抗原SSX2在肝細胞癌組織中的錶達。方法應用RT-PCR技術,對40例肝細胞癌的癌組織及相應癌徬組織進行SSX2基因的mRNA檢測。結果肝細胞癌組織中SSX2 mRNA錶達暘性率為27.5%(11/40),而相應的癌徬組織無SSX2 mRNA錶達暘性率為0(0/40),兩組比較差異有統計學意義(P<0.001)。統計學分析顯示SSX2錶達與AFP暘性率、HbsAg暘性率、病理分級、肝細胞癌腫瘤大小、肝細胞癌是否伴有肝硬化的情況等相關的臨床指標均無關(P>0.05)。結論 SSX2在HCC中呈低頻率的錶達。
목적:검측암-고환항원SSX2재간세포암조직중적표체。방법응용RT-PCR기술,대40례간세포암적암조직급상응암방조직진행SSX2기인적mRNA검측。결과간세포암조직중SSX2 mRNA표체양성솔위27.5%(11/40),이상응적암방조직무SSX2 mRNA표체양성솔위0(0/40),량조비교차이유통계학의의(P<0.001)。통계학분석현시SSX2표체여AFP양성솔、HbsAg양성솔、병리분급、간세포암종류대소、간세포암시부반유간경화적정황등상관적림상지표균무관(P>0.05)。결론 SSX2재HCC중정저빈솔적표체。
Objective Detection of cancer-testis antigen SSX2 expression in HCC tissues. Methods By RT-PCR technique, 40 cases of hepatocellular carcinoma and corresponding adjacent tissues SSX2 gene mRNA detection. Results HCC SSX2mRNA expression was 27.5%(11/40), while the corresponding adjacent tissues without SSX2mRNA expression was 0 (0/40), the difference between the two groups was significant (P<0.01), Statistical analysis showed clinically relevant indicators SSX2 expression and AFP-positive rate, HbsAg positive rate, histological grade, tumor size of hepatocellular carcinoma, hepatocellular carcinoma is associated with cirrhosis of the situation and so are unrelated (P>0.05). Conclusion SSX2 expression in HCC is low frequency.