山西医药杂志
山西醫藥雜誌
산서의약잡지
SHANXI MEDICAL JOURNAL
2014年
6期
611-614
,共4页
癌 ,非小细胞肺%诊断%肺特异X蛋白%细胞角蛋白19%微转移%RT-PCR
癌 ,非小細胞肺%診斷%肺特異X蛋白%細胞角蛋白19%微轉移%RT-PCR
암 ,비소세포폐%진단%폐특이X단백%세포각단백19%미전이%RT-PCR
Carcinoma,Non-small cell lung%Diagnosis%Lunx mRNA%Cytokeratin 19 mRNA%Mi-cro metastasis%Reverse transcriptase polymerase chain reaction
目的检测非小细胞肺癌（NSCLC）患者外周血中肺特异性X蛋白（ Lunx）mRNA、细胞角蛋白19（CK19）mRNA的表达对NSCLC微转移的诊断价值及临床意义。方法采用荧光定量反转录-聚合酶链反应（RT-PCR）技术分别对50例NSCLC患者、15例肺良性疾病（BLD）患者和10名健康人外周血中Lunx mRNA和CK19 mRNA的表达进行检测分析。结果 Lunx mRNA、CK19 mRNA在NSCLC外周血中的阳性表达率分别70％、68％，与其他2组比较差异均有统计学意义（ P＜0.05）。2种标志物共同阳性表达者有28例，阳性率为56％，2种标志物检测比较，差异无统计学意义。Lunx mRNA、CK19 mRNA在 NSCLC 患者阳性表达率与年龄、肿瘤大小、肿瘤部位、病理类型均无关（P＞0.05），而与不同临床分期、组织分化程度及有无淋巴结转移有关（P＜0.05）。结论 Lunx mRNA、CK19 mRNA的高表达可能与NSCLC的微转移有关，可作为检测NSCLC微转移的良好指标，联合检测有助于提高检测的准确度。
目的檢測非小細胞肺癌（NSCLC）患者外週血中肺特異性X蛋白（ Lunx）mRNA、細胞角蛋白19（CK19）mRNA的錶達對NSCLC微轉移的診斷價值及臨床意義。方法採用熒光定量反轉錄-聚閤酶鏈反應（RT-PCR）技術分彆對50例NSCLC患者、15例肺良性疾病（BLD）患者和10名健康人外週血中Lunx mRNA和CK19 mRNA的錶達進行檢測分析。結果 Lunx mRNA、CK19 mRNA在NSCLC外週血中的暘性錶達率分彆70％、68％，與其他2組比較差異均有統計學意義（ P＜0.05）。2種標誌物共同暘性錶達者有28例，暘性率為56％，2種標誌物檢測比較，差異無統計學意義。Lunx mRNA、CK19 mRNA在 NSCLC 患者暘性錶達率與年齡、腫瘤大小、腫瘤部位、病理類型均無關（P＞0.05），而與不同臨床分期、組織分化程度及有無淋巴結轉移有關（P＜0.05）。結論 Lunx mRNA、CK19 mRNA的高錶達可能與NSCLC的微轉移有關，可作為檢測NSCLC微轉移的良好指標，聯閤檢測有助于提高檢測的準確度。
목적검측비소세포폐암（NSCLC）환자외주혈중폐특이성X단백（ Lunx）mRNA、세포각단백19（CK19）mRNA적표체대NSCLC미전이적진단개치급림상의의。방법채용형광정량반전록-취합매련반응（RT-PCR）기술분별대50례NSCLC환자、15례폐량성질병（BLD）환자화10명건강인외주혈중Lunx mRNA화CK19 mRNA적표체진행검측분석。결과 Lunx mRNA、CK19 mRNA재NSCLC외주혈중적양성표체솔분별70％、68％，여기타2조비교차이균유통계학의의（ P＜0.05）。2충표지물공동양성표체자유28례，양성솔위56％，2충표지물검측비교，차이무통계학의의。Lunx mRNA、CK19 mRNA재 NSCLC 환자양성표체솔여년령、종류대소、종류부위、병리류형균무관（P＞0.05），이여불동림상분기、조직분화정도급유무림파결전이유관（P＜0.05）。결론 Lunx mRNA、CK19 mRNA적고표체가능여NSCLC적미전이유관，가작위검측NSCLC미전이적량호지표，연합검측유조우제고검측적준학도。
Objective To detect the expression of Lunx mRNA and CK19 mRNA in peripheral blood of pa-tients with non-small cell lung cancer ,and to explore the clinical and diagnostic value to the micro-metastasis of NSCLC .Methods The expressions of Lunx mRNA and CK19 mRNA were analyzed by reverse transcriptase-poly-merase chain reaction (RT-PCR) in peripheral blood of 50 NSCLC patients ,15 benign lung disease(BLD) and 10 healthy volunteers .Results The positive expression rate of Lunx mRNA and CK 19 mRNA was 70% ,68% in NSCLC respectively .The positive rate was of statistical difference between NSCLC and the others groups (P<0.05).Two markers were in common positive expression in 28 cases ,and the positive rate was 56% .Compared with two markers ,there was no significant difference .The positive expression of Lunx mRNA and CK19 mRNA was of no relationship with age ,tumor size ,tumor location and pathological type (P>0.05) .There was close rela-tionship with the clinical stage ,the extent of tissue differentiation and lymph node metastasis of NSCLC patients ( P<0.05) .Conclusion High expression of Lunx mRNA and CK19 mRNA may be associated with micro-metastasis of NSCLC .Lunx mRNA and CK19 mRNA are good detection markers for micro-metastasis of NSCLC ,and combined detection could improve the positive rate of NSCLC.
