中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2014年
9期
26-28
,共3页
阳离子脂质体%血管抑素基因%Lewis肺癌%小鼠移植瘤
暘離子脂質體%血管抑素基因%Lewis肺癌%小鼠移植瘤
양리자지질체%혈관억소기인%Lewis폐암%소서이식류
Cationic liposomes%Angiostatin gene%Lewis lung cancer%Mice trausplanted
目的：观察血管抑素基因对Lewis肺癌小鼠移植瘤生长、转移的抑制作用。方法：建立C57BL/6j小鼠肺癌模型，30只荷瘤鼠按照随机数字表法分为生理盐水空白对照组、阳离子脂质体对照组、血管抑素基因（PAng）治疗组，每组10只。以阳离子脂质体为介导，将血管抑素基因的重组质粒直接注入移植瘤内，每周3次，共5周，每周测瘤体大小1次，第7周末处死所有小鼠，观察瘤体大小变化、肺表面转移灶数、小鼠生存期等。结果：治疗组小鼠移植瘤生长速度减慢，瘤体缩小，肺内转移少，与对照组比较差异有统计学意义（P<0.05），小鼠活动能力、饮食、对外界刺激的反应能力均无明显改变，生存期明显长于对照组（P<0.05）。结论：阳离子脂质体介导血管抑素基因可有效地抑制Lewis肺癌移植瘤的生长、转移。
目的：觀察血管抑素基因對Lewis肺癌小鼠移植瘤生長、轉移的抑製作用。方法：建立C57BL/6j小鼠肺癌模型，30隻荷瘤鼠按照隨機數字錶法分為生理鹽水空白對照組、暘離子脂質體對照組、血管抑素基因（PAng）治療組，每組10隻。以暘離子脂質體為介導，將血管抑素基因的重組質粒直接註入移植瘤內，每週3次，共5週，每週測瘤體大小1次，第7週末處死所有小鼠，觀察瘤體大小變化、肺錶麵轉移竈數、小鼠生存期等。結果：治療組小鼠移植瘤生長速度減慢，瘤體縮小，肺內轉移少，與對照組比較差異有統計學意義（P<0.05），小鼠活動能力、飲食、對外界刺激的反應能力均無明顯改變，生存期明顯長于對照組（P<0.05）。結論：暘離子脂質體介導血管抑素基因可有效地抑製Lewis肺癌移植瘤的生長、轉移。
목적：관찰혈관억소기인대Lewis폐암소서이식류생장、전이적억제작용。방법：건립C57BL/6j소서폐암모형，30지하류서안조수궤수자표법분위생리염수공백대조조、양리자지질체대조조、혈관억소기인（PAng）치료조，매조10지。이양리자지질체위개도，장혈관억소기인적중조질립직접주입이식류내，매주3차，공5주，매주측류체대소1차，제7주말처사소유소서，관찰류체대소변화、폐표면전이조수、소서생존기등。결과：치료조소서이식류생장속도감만，류체축소，폐내전이소，여대조조비교차이유통계학의의（P<0.05），소서활동능력、음식、대외계자격적반응능력균무명현개변，생존기명현장우대조조（P<0.05）。결론：양리자지질체개도혈관억소기인가유효지억제Lewis폐암이식류적생장、전이。
Objective:To explore the inhibitory effect on the growth and metastasis of tumor by cationic liposomes complex plasmids encoding angiostatin in treatment of mice Lewis lung cancer model. Method:The Lewis lung cancer cell were implanted into 30 C57BL/6j mice to established mice model,and then divided them into the normal saline control group,the cationic liposome group,the PAng group,10 rats in each group. Cationic liposome complex plasmids encoding angiostatin were injected into tumor to inhibit the growth and metastasis of the tumor,3 times a week,for 5 weeks,tumors size measurement 1 time a week,all the mice were sacrificed at the end of the seventh week. The size change of the tumor, the number of long surface metastases,survival period of the mice were observed.Result:The treatment group could inhibit the growth and metastasis of the implanted tumor. Comparing with the control group,they showed significance in tumor size, metastasis in lung and survival period of the mice(P<0.05). Mice activity,diet,and responses to external stimuli ability had no obvious change,and the lifetime of the treatment group was obviously longer than the control group(P<0.05). Conclusion:Cationic liposome complex plasmids encoding angiostatin can inhibit the growth and metastasis of implanted Lewis lung cancer in mice model.
