CAJ | 학술논문

目的：观察急性脑缺血大鼠脑组织中mGluR5 mRNA在不同时段的表达变化的规律，探讨mGluR5 mRNA与脑梗死的相关性。方法：75只雄性Wistar大鼠按照随机数字表法分为正常对照组、手术组1、3、6、12、24 h及3 d、14 d组及其相对应的假手术对照组，采用大鼠MCAO模型，利用原位杂交技术，检测各组mGluR5 mRNA的表达。结果：与正常对照组相比，假手术对照组mGluR5 mRNA表达无明显改变（P>0.05）。手术组mGluR5 mRNA各时间段表达均显著高于假手术对照组及正常对照组，缺血1 h其表达即有升高，比较差异均有统计学意义（P<0.01）。mGluR5 mRNA表达6 h达到高峰（F=5.328，P<0.00），3 d后表达开始下降（P<0.01），14 d基本降至正常对照水平（P>0.05）。结论：mGluR5在脑缺血后升高，提示mGluR5在脑梗死中有着重要作用，从而对我们的临床研究指出一条途径。
목적：관찰급성뇌결혈대서뇌조직중mGluR5 mRNA재불동시단적표체변화적규률，탐토mGluR5 mRNA여뇌경사적상관성。방법：75지웅성Wistar대서안조수궤수자표법분위정상대조조、수술조1、3、6、12、24 h급3 d、14 d조급기상대응적가수술대조조，채용대서MCAO모형，이용원위잡교기술，검측각조mGluR5 mRNA적표체。결과：여정상대조조상비，가수술대조조mGluR5 mRNA표체무명현개변（P>0.05）。수술조mGluR5 mRNA각시간단표체균현저고우가수술대조조급정상대조조，결혈1 h기표체즉유승고，비교차이균유통계학의의（P<0.01）。mGluR5 mRNA표체6 h체도고봉（F=5.328，P<0.00），3 d후표체개시하강（P<0.01），14 d기본강지정상대조수평（P>0.05）。결론：mGluR5재뇌결혈후승고，제시mGluR5재뇌경사중유착중요작용，종이대아문적림상연구지출일조도경。
Objective:To investigate the correlation between mGluR 5mRNA and cerebral infarction.Method:Seventy-five male Wistar rats were randomly divided into normal control group,operation group 1,3,6,12,24 h and 3 d, 14 d groups and corresponding sham operation control group,the rat MCAO model was used in situ hybridization,was used to detect the expression of mGluR5 mRNA.Result:The normal group and the sham operation group had no difference at each time(P>0.05). Each time the operation group mGluR5 mRNA expression were higher than the sham operation control group and normal control group,ischemia 1 h and its expression is increased,and the difference were all statistically significant (P<0.05). The mGluR5 mRNA expression reached peak at 6 h(F=5.328,P<0.00),began to decline after 3 d(P<0.01), the basic dropped to normal control level after 14 d(P>0.05).Conclusion:MGluR5 increased after cerebral ischemia, suggesting that mGluR5 plays an important role in cerebral infarction,so as to point out a way for our clinical study.